Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18257 | 3' | -53.7 | NC_004681.1 | + | 13007 | 0.66 | 0.893404 |
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Target: 5'- aCCGCGGaCGa-CGUGGcCGCGACuGAc -3' miRNA: 3'- -GGCGCCaGCaaGUACUuGCGCUGcCU- -5' |
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| 18257 | 3' | -53.7 | NC_004681.1 | + | 12397 | 0.72 | 0.566503 |
|
Target: 5'- aCCGCGGcgacgcUCGUcgGUGAGCcgGUGACGGAc -3' miRNA: 3'- -GGCGCC------AGCAagUACUUG--CGCUGCCU- -5' |
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| 18257 | 3' | -53.7 | NC_004681.1 | + | 10617 | 0.67 | 0.836657 |
|
Target: 5'- uCCGCGGUCGgacuUCGcgcccuugccgaucUGGACaaagGUGACGGu -3' miRNA: 3'- -GGCGCCAGCa---AGU--------------ACUUG----CGCUGCCu -5' |
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| 18257 | 3' | -53.7 | NC_004681.1 | + | 7143 | 1.11 | 0.002015 |
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Target: 5'- gCCGCGGUCGUUCAUGAACGCGACGGAg -3' miRNA: 3'- -GGCGCCAGCAAGUACUUGCGCUGCCU- -5' |
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| 18257 | 3' | -53.7 | NC_004681.1 | + | 6797 | 0.68 | 0.830605 |
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Target: 5'- aCCGCGGUgcucucaacccCGUcgAUGGAcCGUGACGGc -3' miRNA: 3'- -GGCGCCA-----------GCAagUACUU-GCGCUGCCu -5' |
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| 18257 | 3' | -53.7 | NC_004681.1 | + | 6371 | 0.74 | 0.48466 |
|
Target: 5'- cUCGCGGcccUCGUUCggGGugGCGAcacCGGAg -3' miRNA: 3'- -GGCGCC---AGCAAGuaCUugCGCU---GCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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