Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18260 | 3' | -55.3 | NC_004681.1 | + | 62714 | 0.7 | 0.556458 |
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Target: 5'- -cGGACgggGUGggGGCGuagaugaccuccaaAGCAUCcCUCCg -3' miRNA: 3'- gaCCUG---CGCuuCCGC--------------UCGUAGaGAGG- -5' |
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| 18260 | 3' | -55.3 | NC_004681.1 | + | 27808 | 0.71 | 0.538671 |
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Target: 5'- aUGGGCGCGgcGGCGccaacgguGGCGcauccgucuUCUCaUCCg -3' miRNA: 3'- gACCUGCGCuuCCGC--------UCGU---------AGAG-AGG- -5' |
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| 18260 | 3' | -55.3 | NC_004681.1 | + | 991 | 0.71 | 0.528299 |
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Target: 5'- -cGGACGCcAGGGCGGcuucccGC-UCUCUCUg -3' miRNA: 3'- gaCCUGCGcUUCCGCU------CGuAGAGAGG- -5' |
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| 18260 | 3' | -55.3 | NC_004681.1 | + | 23476 | 0.71 | 0.507785 |
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Target: 5'- aCUGGcgaACGUGGAGGCGGGCAggaggUUgaCCa -3' miRNA: 3'- -GACC---UGCGCUUCCGCUCGUa----GAgaGG- -5' |
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| 18260 | 3' | -55.3 | NC_004681.1 | + | 13358 | 0.75 | 0.326726 |
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Target: 5'- cCUGGACGCGGAauucGGCGAGCuugacgcggcgGUCgcgCUUCu -3' miRNA: 3'- -GACCUGCGCUU----CCGCUCG-----------UAGa--GAGG- -5' |
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| 18260 | 3' | -55.3 | NC_004681.1 | + | 62962 | 0.77 | 0.249523 |
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Target: 5'- gUGGugGCGGcggcGGcGCGAGCAgCUUUCCg -3' miRNA: 3'- gACCugCGCU----UC-CGCUCGUaGAGAGG- -5' |
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| 18260 | 3' | -55.3 | NC_004681.1 | + | 39286 | 0.8 | 0.156184 |
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Target: 5'- cCUcGACGuCGAGGGCGAGCAUCUCcgcggCCc -3' miRNA: 3'- -GAcCUGC-GCUUCCGCUCGUAGAGa----GG- -5' |
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| 18260 | 3' | -55.3 | NC_004681.1 | + | 8072 | 1.04 | 0.003506 |
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Target: 5'- aCUGG-CGCGAAGGCGAGCAUCUCUCCg -3' miRNA: 3'- -GACCuGCGCUUCCGCUCGUAGAGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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