Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18260 | 5' | -52 | NC_004681.1 | + | 17949 | 0.71 | 0.686081 |
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Target: 5'- --uGCcGAGGGcGGCUCcgUgGGCCACCg -3' miRNA: 3'- guuUGuCUCUC-CCGAGa-AgCUGGUGG- -5' |
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| 18260 | 5' | -52 | NC_004681.1 | + | 2978 | 0.72 | 0.66424 |
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Target: 5'- uCGAGCGGgaGGGGGGCUUcgucacggUCGAgCGCCu -3' miRNA: 3'- -GUUUGUC--UCUCCCGAGa-------AGCUgGUGG- -5' |
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| 18260 | 5' | -52 | NC_004681.1 | + | 54644 | 0.72 | 0.653269 |
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Target: 5'- cCGGGCGGAugauGaAGGGCUCUgcggugaucUCGgGCCACCg -3' miRNA: 3'- -GUUUGUCU----C-UCCCGAGA---------AGC-UGGUGG- -5' |
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| 18260 | 5' | -52 | NC_004681.1 | + | 62521 | 0.73 | 0.565625 |
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Target: 5'- -cGGCGGAGAGGGCggugUCGGCgcccaGCCa -3' miRNA: 3'- guUUGUCUCUCCCGaga-AGCUGg----UGG- -5' |
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| 18260 | 5' | -52 | NC_004681.1 | + | 9607 | 0.74 | 0.554823 |
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Target: 5'- -cAGCAGGccGAGGcGCUCUUCGGCgCGCa -3' miRNA: 3'- guUUGUCU--CUCC-CGAGAAGCUG-GUGg -5' |
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| 18260 | 5' | -52 | NC_004681.1 | + | 20555 | 0.75 | 0.45125 |
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Target: 5'- -cAGCGGAGuuAGGGCgg--CGACCACCu -3' miRNA: 3'- guUUGUCUC--UCCCGagaaGCUGGUGG- -5' |
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| 18260 | 5' | -52 | NC_004681.1 | + | 8039 | 1.13 | 0.001813 |
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Target: 5'- uCAAACAGAGAGGGCUCUUCGACCACCa -3' miRNA: 3'- -GUUUGUCUCUCCCGAGAAGCUGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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