Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18262 | 3' | -53.5 | NC_004681.1 | + | 67034 | 0.66 | 0.869333 |
Target: 5'- gCGCGCGGcaccagGGCC--GCCAcagagUCAACGAc -3' miRNA: 3'- -GCGUGCCa-----CUGGaaCGGU-----AGUUGCUu -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 41003 | 0.66 | 0.869333 |
Target: 5'- aCGUccucaAUGGUGGgCUUGCCAUCcaucGCGGc -3' miRNA: 3'- -GCG-----UGCCACUgGAACGGUAGu---UGCUu -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 3380 | 0.66 | 0.85209 |
Target: 5'- gGCGCGGgacuccuugagggUGGCCUU-CCAUCcgAGCGAGc -3' miRNA: 3'- gCGUGCC-------------ACUGGAAcGGUAG--UUGCUU- -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 14495 | 0.66 | 0.844384 |
Target: 5'- gCGCGCuGGUGGCCggUGCUggC-GCGAGc -3' miRNA: 3'- -GCGUG-CCACUGGa-ACGGuaGuUGCUU- -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 29143 | 0.66 | 0.835611 |
Target: 5'- gGCGUGGcGACCUcaacgGCCAcgUCAACGAc -3' miRNA: 3'- gCGUGCCaCUGGAa----CGGU--AGUUGCUu -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 5767 | 0.67 | 0.826623 |
Target: 5'- aGCGgauCGGcacGACCUcGUCGUCGGCGAAg -3' miRNA: 3'- gCGU---GCCa--CUGGAaCGGUAGUUGCUU- -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 32507 | 0.67 | 0.817431 |
Target: 5'- uGCGCGGUggugccuucguaGugCUUGCCGUUGugGu- -3' miRNA: 3'- gCGUGCCA------------CugGAACGGUAGUugCuu -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 9183 | 0.67 | 0.798473 |
Target: 5'- gCGC-CGGUGACgacgcgGCCAuacuUCAGCGAc -3' miRNA: 3'- -GCGuGCCACUGgaa---CGGU----AGUUGCUu -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 17380 | 0.68 | 0.778819 |
Target: 5'- aGCGCGGUcgagGACCUcGUCAacaUCAugGAc -3' miRNA: 3'- gCGUGCCA----CUGGAaCGGU---AGUugCUu -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 32214 | 0.68 | 0.748227 |
Target: 5'- cCGcCGCGGUGACCgcuuccuggaUGCCggUGACGAc -3' miRNA: 3'- -GC-GUGCCACUGGa---------ACGGuaGUUGCUu -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 38985 | 0.68 | 0.727223 |
Target: 5'- gCGCugGGUGGCCgacgaggaaGgCGUCAACGu- -3' miRNA: 3'- -GCGugCCACUGGaa-------CgGUAGUUGCuu -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 41207 | 0.68 | 0.727223 |
Target: 5'- aGCACGGUGACCguccgagUGUgAcCAACGc- -3' miRNA: 3'- gCGUGCCACUGGa------ACGgUaGUUGCuu -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 12092 | 0.68 | 0.727223 |
Target: 5'- gGCuuGGUGGCCUUGUagcCAGCGGu -3' miRNA: 3'- gCGugCCACUGGAACGguaGUUGCUu -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 9092 | 0.69 | 0.716573 |
Target: 5'- uGCugACGGUGGCCgagguggcGCCGaCAGCGAGg -3' miRNA: 3'- gCG--UGCCACUGGaa------CGGUaGUUGCUU- -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 48802 | 0.69 | 0.70584 |
Target: 5'- gGCGCgGGUGACaucugauggUUGCCGUCGACa-- -3' miRNA: 3'- gCGUG-CCACUGg--------AACGGUAGUUGcuu -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 12188 | 0.69 | 0.70584 |
Target: 5'- aCGCGCGGgcgGACCUcgGCCAcguacUCGGCc-- -3' miRNA: 3'- -GCGUGCCa--CUGGAa-CGGU-----AGUUGcuu -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 5647 | 0.69 | 0.673252 |
Target: 5'- aCGCAgcUGGUGAgCUUcGCCGaCGACGAGg -3' miRNA: 3'- -GCGU--GCCACUgGAA-CGGUaGUUGCUU- -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 20011 | 0.7 | 0.651314 |
Target: 5'- gGCccGCGGUGGCCUucaUGCCGUgcccggugUAGCGAu -3' miRNA: 3'- gCG--UGCCACUGGA---ACGGUA--------GUUGCUu -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 62365 | 0.7 | 0.640314 |
Target: 5'- --gGCGGUGAUCUUGCCcccggcgcgGUgGACGAAg -3' miRNA: 3'- gcgUGCCACUGGAACGG---------UAgUUGCUU- -5' |
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18262 | 3' | -53.5 | NC_004681.1 | + | 39029 | 0.7 | 0.6183 |
Target: 5'- uGCACGGUGGCCgguugcagGCUGcCAugGAGu -3' miRNA: 3'- gCGUGCCACUGGaa------CGGUaGUugCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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