Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18269 | 5' | -56.3 | NC_004681.1 | + | 74399 | 0.66 | 0.727124 |
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Target: 5'- cGCUUcgaUCUGCacucGGAagucaccuuccGGGGCGGuccagccACGUCGCu -3' miRNA: 3'- -UGGA---AGACG----UCU-----------UCCCGCC-------UGCAGCG- -5' |
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| 18269 | 5' | -56.3 | NC_004681.1 | + | 35826 | 0.66 | 0.748536 |
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Target: 5'- uCCgUCUGagaguucgaacCGGAuGGGUGGGCgGUCGCu -3' miRNA: 3'- uGGaAGAC-----------GUCUuCCCGCCUG-CAGCG- -5' |
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| 18269 | 5' | -56.3 | NC_004681.1 | + | 37656 | 0.66 | 0.748536 |
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Target: 5'- -aCUUCUGCGGGgccucgccAGcGGcCGcGCGUCGCu -3' miRNA: 3'- ugGAAGACGUCU--------UC-CC-GCcUGCAGCG- -5' |
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| 18269 | 5' | -56.3 | NC_004681.1 | + | 26331 | 0.66 | 0.758565 |
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Target: 5'- cACCgc--GUAG-GGGGCGGGCaUCGCc -3' miRNA: 3'- -UGGaagaCGUCuUCCCGCCUGcAGCG- -5' |
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| 18269 | 5' | -56.3 | NC_004681.1 | + | 50295 | 0.66 | 0.767488 |
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Target: 5'- cACCUUggggugcUUGUGGAAGuugcGGCuaaccuGGACGUCGCu -3' miRNA: 3'- -UGGAA-------GACGUCUUC----CCG------CCUGCAGCG- -5' |
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| 18269 | 5' | -56.3 | NC_004681.1 | + | 57905 | 0.66 | 0.768473 |
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Target: 5'- uCCUUCgccaccucgGCGGcgcGGCGcGugGUCGCg -3' miRNA: 3'- uGGAAGa--------CGUCuucCCGC-CugCAGCG- -5' |
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| 18269 | 5' | -56.3 | NC_004681.1 | + | 7419 | 0.66 | 0.768473 |
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Target: 5'- aGCCccg-GguGccGGGGGUGGAaCGUCGCu -3' miRNA: 3'- -UGGaagaCguC--UUCCCGCCU-GCAGCG- -5' |
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| 18269 | 5' | -56.3 | NC_004681.1 | + | 56136 | 0.66 | 0.768473 |
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Target: 5'- aGCUgcgUCUccacGguGAAGGcgauGCGGAgGUCGCc -3' miRNA: 3'- -UGGa--AGA----CguCUUCC----CGCCUgCAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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