miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
18274 3' -55.7 NC_004681.1 + 21225 0.69 0.597241
Target:  5'- -gGGGACCACCAcaaUGGGcgUCAUgAGCg- -3'
miRNA:   3'- ggCUCUGGUGGU---ACCC--AGUAgUCGgu -5'
18274 3' -55.7 NC_004681.1 + 49325 0.71 0.513514
Target:  5'- gCCGGuGACCAagacgaCGcGGGUCAcuUCAGCCGu -3'
miRNA:   3'- -GGCU-CUGGUg-----GUaCCCAGU--AGUCGGU- -5'
18274 3' -55.7 NC_004681.1 + 28752 0.72 0.434985
Target:  5'- uUGAGACCgACCGUGGGUCcgauaguUCcGCCu -3'
miRNA:   3'- gGCUCUGG-UGGUACCCAGu------AGuCGGu -5'
18274 3' -55.7 NC_004681.1 + 15914 0.72 0.416451
Target:  5'- aCCGAG-CCACUGUGcgacguugcggaGGcCGUCAGCCAu -3'
miRNA:   3'- -GGCUCuGGUGGUAC------------CCaGUAGUCGGU- -5'
18274 3' -55.7 NC_004681.1 + 62571 0.8 0.142835
Target:  5'- uUCGuAGACCACCGUgGGGUCGugccccUCGGCCAu -3'
miRNA:   3'- -GGC-UCUGGUGGUA-CCCAGU------AGUCGGU- -5'
18274 3' -55.7 NC_004681.1 + 16534 1.1 0.001127
Target:  5'- gCCGAGACCACCAUGGGUCAUCAGCCAg -3'
miRNA:   3'- -GGCUCUGGUGGUACCCAGUAGUCGGU- -5'
18274 3' -55.7 NC_004681.1 + 1419 0.66 0.802644
Target:  5'- uUGAGGCaGCCcuUGucggcgaGGUCGUCGGCCAg -3'
miRNA:   3'- gGCUCUGgUGGu-AC-------CCAGUAGUCGGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.