Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18278 | 5' | -54.2 | NC_004681.1 | + | 62479 | 0.66 | 0.857494 |
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Target: 5'- cCCACgguggucuaCGAACUccugaUCAUGGCCCaGGccaUGGg -3' miRNA: 3'- cGGUG---------GCUUGA-----AGUACCGGG-CCa--ACC- -5' |
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| 18278 | 5' | -54.2 | NC_004681.1 | + | 66842 | 0.66 | 0.857494 |
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Target: 5'- uGCCACCGGcCUUCugcgcauUGGCCaccgcgUGGa -3' miRNA: 3'- -CGGUGGCUuGAAGu------ACCGGgcca--ACC- -5' |
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| 18278 | 5' | -54.2 | NC_004681.1 | + | 38375 | 0.66 | 0.865489 |
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Target: 5'- cGCCaagugacguACCGccUUUCGUGaGCCCGGagGGu -3' miRNA: 3'- -CGG---------UGGCuuGAAGUAC-CGGGCCaaCC- -5' |
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| 18278 | 5' | -54.2 | NC_004681.1 | + | 17635 | 0.66 | 0.873252 |
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Target: 5'- uGCCGCCaGGGag-CGUGGCCUGcaugUGGc -3' miRNA: 3'- -CGGUGG-CUUgaaGUACCGGGCca--ACC- -5' |
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| 18278 | 5' | -54.2 | NC_004681.1 | + | 60000 | 0.66 | 0.873252 |
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Target: 5'- uGCCugCGggUccUCAa-GCCCGGU-GGg -3' miRNA: 3'- -CGGugGCuuGa-AGUacCGGGCCAaCC- -5' |
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| 18278 | 5' | -54.2 | NC_004681.1 | + | 67117 | 0.66 | 0.880778 |
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Target: 5'- uGCCAgUGAGCUugcgCAUGGCCUGcGa--- -3' miRNA: 3'- -CGGUgGCUUGAa---GUACCGGGC-Caacc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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