Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18344 | 5' | -55.9 | NC_004681.1 | + | 26243 | 0.71 | 0.546192 |
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Target: 5'- cCCCCuacgcggugggGCAUGACGGAcucCUCGGCGg -3' miRNA: 3'- -GGGGca---------UGUGCUGCCUcuuGAGCCGC- -5' |
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| 18344 | 5' | -55.9 | NC_004681.1 | + | 61858 | 0.71 | 0.517828 |
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Target: 5'- aCUUCGUugACGGCGuGaAGAcuACUCGGUGg -3' miRNA: 3'- -GGGGCAugUGCUGC-C-UCU--UGAGCCGC- -5' |
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| 18344 | 5' | -55.9 | NC_004681.1 | + | 25772 | 0.72 | 0.497932 |
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Target: 5'- aCCCCGgACAUGcacuuuCGGAucGGCUCGGCGa -3' miRNA: 3'- -GGGGCaUGUGCu-----GCCUc-UUGAGCCGC- -5' |
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| 18344 | 5' | -55.9 | NC_004681.1 | + | 61231 | 0.73 | 0.440478 |
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Target: 5'- gUUCGUuggACACGACGGuGAAgUUGGCGa -3' miRNA: 3'- gGGGCA---UGUGCUGCCuCUUgAGCCGC- -5' |
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| 18344 | 5' | -55.9 | NC_004681.1 | + | 13182 | 0.73 | 0.440478 |
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Target: 5'- gCgCUGUACuagacaGCGGCGGcuucGAGCUCGGCGa -3' miRNA: 3'- -GgGGCAUG------UGCUGCCu---CUUGAGCCGC- -5' |
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| 18344 | 5' | -55.9 | NC_004681.1 | + | 13910 | 0.73 | 0.425799 |
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Target: 5'- cCCCCGUACACGAUguugagcaagcgccgGGAcGAcaGCUUGGgGg -3' miRNA: 3'- -GGGGCAUGUGCUG---------------CCU-CU--UGAGCCgC- -5' |
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| 18344 | 5' | -55.9 | NC_004681.1 | + | 46715 | 0.75 | 0.345799 |
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Target: 5'- gCgUGUGCGgGGCGGGGucCUCGGCGa -3' miRNA: 3'- gGgGCAUGUgCUGCCUCuuGAGCCGC- -5' |
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| 18344 | 5' | -55.9 | NC_004681.1 | + | 24973 | 0.75 | 0.337948 |
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Target: 5'- cCCCCG---GCGACGG-GGGCUCuGGCGg -3' miRNA: 3'- -GGGGCaugUGCUGCCuCUUGAG-CCGC- -5' |
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| 18344 | 5' | -55.9 | NC_004681.1 | + | 67376 | 1.1 | 0.001541 |
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Target: 5'- uCCCCGUACACGACGGAGAACUCGGCGu -3' miRNA: 3'- -GGGGCAUGUGCUGCCUCUUGAGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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