Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18524 | 3' | -53 | NC_004681.1 | + | 14572 | 0.73 | 0.563011 |
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Target: 5'- -cGCCCUGCUcggcGGAG-GCGGCGcgcgCGGCg -3' miRNA: 3'- guCGGGAUGA----CUUCuUGCUGCa---GCCG- -5' |
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| 18524 | 3' | -53 | NC_004681.1 | + | 15011 | 0.73 | 0.552338 |
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Target: 5'- aAGgCgUACUGGAacugcGAgGCGACGUCGGCg -3' miRNA: 3'- gUCgGgAUGACUU-----CU-UGCUGCAGCCG- -5' |
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| 18524 | 3' | -53 | NC_004681.1 | + | 3238 | 0.73 | 0.541727 |
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Target: 5'- aGGCCCUGgaGGAGGGCGAgcCGcUgGGCg -3' miRNA: 3'- gUCGGGAUgaCUUCUUGCU--GC-AgCCG- -5' |
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| 18524 | 3' | -53 | NC_004681.1 | + | 49652 | 0.75 | 0.450041 |
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Target: 5'- uCGGgCCUGCccgcgGggGAaggccaggGCGAUGUCGGCa -3' miRNA: 3'- -GUCgGGAUGa----CuuCU--------UGCUGCAGCCG- -5' |
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| 18524 | 3' | -53 | NC_004681.1 | + | 30407 | 0.75 | 0.440372 |
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Target: 5'- -cGCUUUAUcGGAGAGCG-CGUCGGCa -3' miRNA: 3'- guCGGGAUGaCUUCUUGCuGCAGCCG- -5' |
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| 18524 | 3' | -53 | NC_004681.1 | + | 67675 | 1.12 | 0.00161 |
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Target: 5'- uCAGCCCUACUGAAGAACGACGUCGGCa -3' miRNA: 3'- -GUCGGGAUGACUUCUUGCUGCAGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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