Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18534 | 3' | -54.9 | NC_004681.1 | + | 61769 | 0.66 | 0.840839 |
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Target: 5'- cUCGGCgGCGGccuccgcggCGGCGGCUUCuGCGc -3' miRNA: 3'- -GGCUGaCGUCaa-------GCUGUCGGAGuCGC- -5' |
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| 18534 | 3' | -54.9 | NC_004681.1 | + | 62072 | 0.66 | 0.840839 |
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Target: 5'- aUGACUgugcGCGGcaUGGCGGCCUCcaugGGCGg -3' miRNA: 3'- gGCUGA----CGUCaaGCUGUCGGAG----UCGC- -5' |
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| 18534 | 3' | -54.9 | NC_004681.1 | + | 63413 | 0.66 | 0.80416 |
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Target: 5'- cUCGACUGCGGUccugaauUCccuGGCcaccaggcuuccAGCCUCGGCc -3' miRNA: 3'- -GGCUGACGUCA-------AG---CUG------------UCGGAGUCGc -5' |
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| 18534 | 3' | -54.9 | NC_004681.1 | + | 67309 | 0.79 | 0.206364 |
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Target: 5'- cCCGGgUGCGGUUgcCGACAGCCUCAucGCc -3' miRNA: 3'- -GGCUgACGUCAA--GCUGUCGGAGU--CGc -5' |
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| 18534 | 3' | -54.9 | NC_004681.1 | + | 68555 | 0.75 | 0.347382 |
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Target: 5'- aCGACgagGuCAGccuucUUCGGCAGCCUCAGUa -3' miRNA: 3'- gGCUGa--C-GUC-----AAGCUGUCGGAGUCGc -5' |
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| 18534 | 3' | -54.9 | NC_004681.1 | + | 70793 | 0.66 | 0.805092 |
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Target: 5'- uCCG-UUG-AG-UCGcAUAGCCUCAGCGg -3' miRNA: 3'- -GGCuGACgUCaAGC-UGUCGGAGUCGC- -5' |
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| 18534 | 3' | -54.9 | NC_004681.1 | + | 72099 | 0.68 | 0.704044 |
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Target: 5'- cUCGAUUGCAGUcuucaugUC-ACAGCC-CAGCc -3' miRNA: 3'- -GGCUGACGUCA-------AGcUGUCGGaGUCGc -5' |
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| 18534 | 3' | -54.9 | NC_004681.1 | + | 73093 | 1.11 | 0.001204 |
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Target: 5'- uCCGACUGCAGUUCGACAGCCUCAGCGg -3' miRNA: 3'- -GGCUGACGUCAAGCUGUCGGAGUCGC- -5' |
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| 18534 | 3' | -54.9 | NC_004681.1 | + | 73119 | 0.68 | 0.725978 |
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Target: 5'- -gGucuCUGCcuccgccUCGACAGCCUCAGCc -3' miRNA: 3'- ggCu--GACGuca----AGCUGUCGGAGUCGc -5' |
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| 18534 | 3' | -54.9 | NC_004681.1 | + | 74215 | 0.66 | 0.814311 |
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Target: 5'- aUGA--GCAacguGUUUGACAGCCUCAuGCGu -3' miRNA: 3'- gGCUgaCGU----CAAGCUGUCGGAGU-CGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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