Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18547 | 3' | -55.9 | NC_004682.1 | + | 17626 | 0.67 | 0.60181 |
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Target: 5'- cGCUGCUGG--UGCCCAaccugcaGACgcUCGGc -3' miRNA: 3'- -CGACGACCuuGCGGGUg------CUGa-AGCCc -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 13598 | 0.73 | 0.307209 |
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Target: 5'- cGCUcCUGGGACGCCgagCACGGCgUUCuGGGc -3' miRNA: 3'- -CGAcGACCUUGCGG---GUGCUG-AAG-CCC- -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 12670 | 0.7 | 0.445695 |
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Target: 5'- --gGCUGGAccgaggGCGUCCACGACUacguccaccaGGGg -3' miRNA: 3'- cgaCGACCU------UGCGGGUGCUGAag--------CCC- -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 10466 | 0.71 | 0.398714 |
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Target: 5'- aGCUGCUGaucGAGCugGCCCAgcgaGGCUUCGcGGu -3' miRNA: 3'- -CGACGAC---CUUG--CGGGUg---CUGAAGC-CC- -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 10078 | 0.66 | 0.667216 |
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Target: 5'- --gGCgUGGggUGCCCGCcag-UCGGGc -3' miRNA: 3'- cgaCG-ACCuuGCGGGUGcugaAGCCC- -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 7150 | 0.66 | 0.678063 |
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Target: 5'- cGCUcgGCUGGGGCGUCC-UGaACUggUGGGc -3' miRNA: 3'- -CGA--CGACCUUGCGGGuGC-UGAa-GCCC- -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 5724 | 0.67 | 0.606164 |
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Target: 5'- aGUUGCUGGcACGaCCACaACUgaacauagaaucuauUCGGGa -3' miRNA: 3'- -CGACGACCuUGCgGGUGcUGA---------------AGCCC- -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 5275 | 0.66 | 0.696398 |
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Target: 5'- cGC-GCUGGGGCugcugcucacgccgGCCC-CGGCgcaggCGGGu -3' miRNA: 3'- -CGaCGACCUUG--------------CGGGuGCUGaa---GCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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