Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18547 | 3' | -55.9 | NC_004682.1 | + | 18451 | 0.66 | 0.667216 |
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Target: 5'- cGCUGCU----CGUCUACGGCgacagCGGGg -3' miRNA: 3'- -CGACGAccuuGCGGGUGCUGaa---GCCC- -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 48826 | 0.66 | 0.667216 |
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Target: 5'- ---cCUGGAcggguGCGCCguCGGCcUCGGGg -3' miRNA: 3'- cgacGACCU-----UGCGGguGCUGaAGCCC- -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 19072 | 0.66 | 0.678063 |
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Target: 5'- cCU-CUGGGGCuUCCGCGACUUCGuccGGg -3' miRNA: 3'- cGAcGACCUUGcGGGUGCUGAAGC---CC- -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 7150 | 0.66 | 0.678063 |
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Target: 5'- cGCUcgGCUGGGGCGUCC-UGaACUggUGGGc -3' miRNA: 3'- -CGA--CGACCUUGCGGGuGC-UGAa-GCCC- -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 47099 | 0.66 | 0.688867 |
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Target: 5'- aCUGCUGGuAUGCCaaaGCGGCguagUCGcGa -3' miRNA: 3'- cGACGACCuUGCGGg--UGCUGa---AGC-Cc -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 5275 | 0.66 | 0.696398 |
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Target: 5'- cGC-GCUGGGGCugcugcucacgccgGCCC-CGGCgcaggCGGGu -3' miRNA: 3'- -CGaCGACCUUG--------------CGGGuGCUGaa---GCCC- -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 48249 | 0.66 | 0.703899 |
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Target: 5'- cCUGCUGGAcccuguggccccugaAUGCCCuGCgGGCcUUGGGc -3' miRNA: 3'- cGACGACCU---------------UGCGGG-UG-CUGaAGCCC- -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 39217 | 0.66 | 0.710302 |
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Target: 5'- --cGCUGGAACGCCuCGCG-CU-CGu- -3' miRNA: 3'- cgaCGACCUUGCGG-GUGCuGAaGCcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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