Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18547 | 3' | -55.9 | NC_004682.1 | + | 24779 | 0.71 | 0.417127 |
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Target: 5'- cGCUaucugGUUGGuACGCCUGCGAacUCGGGu -3' miRNA: 3'- -CGA-----CGACCuUGCGGGUGCUgaAGCCC- -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 10466 | 0.71 | 0.398714 |
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Target: 5'- aGCUGCUGaucGAGCugGCCCAgcgaGGCUUCGcGGu -3' miRNA: 3'- -CGACGAC---CUUG--CGGGUg---CUGAAGC-CC- -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 38446 | 0.71 | 0.389705 |
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Target: 5'- uGCUcCUGGcACGCCCACcg--UCGGGa -3' miRNA: 3'- -CGAcGACCuUGCGGGUGcugaAGCCC- -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 21827 | 0.71 | 0.372091 |
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Target: 5'- cGCUGCUGGAgaccagcgagacGgGCCUggGCGACUUCcacuacuacgaGGGu -3' miRNA: 3'- -CGACGACCU------------UgCGGG--UGCUGAAG-----------CCC- -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 13598 | 0.73 | 0.307209 |
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Target: 5'- cGCUcCUGGGACGCCgagCACGGCgUUCuGGGc -3' miRNA: 3'- -CGAcGACCUUGCGG---GUGCUG-AAG-CCC- -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 32120 | 0.74 | 0.264475 |
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Target: 5'- cGCUGCUGGAGggcUGCCUucGCGGCcuugUCGGu -3' miRNA: 3'- -CGACGACCUU---GCGGG--UGCUGa---AGCCc -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 41962 | 0.82 | 0.077635 |
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Target: 5'- uCUGCUGGGggaccgugACGCCUACGGCgUCGGGc -3' miRNA: 3'- cGACGACCU--------UGCGGGUGCUGaAGCCC- -5' |
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| 18547 | 3' | -55.9 | NC_004682.1 | + | 44925 | 1.12 | 0.000553 |
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Target: 5'- aGCUGCUGGAACGCCCACGACUUCGGGu -3' miRNA: 3'- -CGACGACCUUGCGGGUGCUGAAGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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