Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18547 | 5' | -55.3 | NC_004682.1 | + | 41936 | 0.67 | 0.65181 |
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Target: 5'- -cCGUGGGCAGCGgGUcaccggcagagcccUUGAUGUACa -3' miRNA: 3'- caGCGCUCGUUGCgCG--------------AGCUGCAUGa -5' |
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| 18547 | 5' | -55.3 | NC_004682.1 | + | 35207 | 0.67 | 0.65512 |
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Target: 5'- --gGCGGGCGA-GCGCUCGGCu---- -3' miRNA: 3'- cagCGCUCGUUgCGCGAGCUGcauga -5' |
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| 18547 | 5' | -55.3 | NC_004682.1 | + | 27877 | 0.67 | 0.65512 |
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Target: 5'- -aCGCGAGCGccaggggaGCaGCGa-CGACGUACa -3' miRNA: 3'- caGCGCUCGU--------UG-CGCgaGCUGCAUGa -5' |
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| 18547 | 5' | -55.3 | NC_004682.1 | + | 16195 | 0.66 | 0.708714 |
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Target: 5'- -aCGCGAGguGCuggaucgcaaccgGCGCaUCGGCGUGg- -3' miRNA: 3'- caGCGCUCguUG-------------CGCG-AGCUGCAUga -5' |
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| 18547 | 5' | -55.3 | NC_004682.1 | + | 41817 | 0.66 | 0.709794 |
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Target: 5'- cUCGCGccAGCAGCGCGUaggUCagGACGaagGCUg -3' miRNA: 3'- cAGCGC--UCGUUGCGCG---AG--CUGCa--UGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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