Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18547 | 5' | -55.3 | NC_004682.1 | + | 41936 | 0.67 | 0.65181 |
|
Target: 5'- -cCGUGGGCAGCGgGUcaccggcagagcccUUGAUGUACa -3' miRNA: 3'- caGCGCUCGUUGCgCG--------------AGCUGCAUGa -5' |
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| 18547 | 5' | -55.3 | NC_004682.1 | + | 43642 | 0.72 | 0.337583 |
|
Target: 5'- --gGCGAGcCAGCGUGCUCaccgccgggggucuuGACGUACg -3' miRNA: 3'- cagCGCUC-GUUGCGCGAG---------------CUGCAUGa -5' |
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| 18547 | 5' | -55.3 | NC_004682.1 | + | 44959 | 1.07 | 0.001231 |
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Target: 5'- cGUCGCGAGCAACGCGCUCGACGUACUc -3' miRNA: 3'- -CAGCGCUCGUUGCGCGAGCUGCAUGA- -5' |
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| 18547 | 5' | -55.3 | NC_004682.1 | + | 45768 | 0.69 | 0.492504 |
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Target: 5'- -gCGgGAGCAGCcgGUGCggcgUCGGCGUACg -3' miRNA: 3'- caGCgCUCGUUG--CGCG----AGCUGCAUGa -5' |
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| 18547 | 5' | -55.3 | NC_004682.1 | + | 45899 | 0.67 | 0.621971 |
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Target: 5'- -gCGCGAGUAGCGCacucggGCUCGcCGU-Cg -3' miRNA: 3'- caGCGCUCGUUGCG------CGAGCuGCAuGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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