Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18555 | 3' | -56.3 | NC_004682.1 | + | 10691 | 0.74 | 0.234976 |
Target: 5'- uGGCGGC-GAUccUgCUCgAUGCUGCCGCa -3' miRNA: 3'- cCCGCCGuCUA--AgGAG-UAUGGCGGCG- -5' |
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18555 | 3' | -56.3 | NC_004682.1 | + | 34507 | 0.76 | 0.166378 |
Target: 5'- gGGcGCGGCcccagUCCuUCAUGCCGUCGCg -3' miRNA: 3'- -CC-CGCCGucua-AGG-AGUAUGGCGGCG- -5' |
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18555 | 3' | -56.3 | NC_004682.1 | + | 15590 | 0.77 | 0.157573 |
Target: 5'- cGGCGGCAcgg-CCUCGggGCCGCUGCc -3' miRNA: 3'- cCCGCCGUcuaaGGAGUa-UGGCGGCG- -5' |
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18555 | 3' | -56.3 | NC_004682.1 | + | 40500 | 1.14 | 0.000364 |
Target: 5'- aGGGCGGCAGAUUCCUCAUACCGCCGCu -3' miRNA: 3'- -CCCGCCGUCUAAGGAGUAUGGCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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