Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18571 | 5' | -59.6 | NC_004682.1 | + | 1883 | 0.7 | 0.26302 |
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Target: 5'- gGCCCUGCgggCGAgGGACUCCacaucgacggccaGGuGGCCGa -3' miRNA: 3'- -CGGGACGa--GCU-UCUGGGG-------------CCuCUGGC- -5' |
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| 18571 | 5' | -59.6 | NC_004682.1 | + | 46766 | 0.71 | 0.257206 |
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Target: 5'- -aCCUGCagcCGgcGGCUuuGGAGACCGc -3' miRNA: 3'- cgGGACGa--GCuuCUGGggCCUCUGGC- -5' |
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| 18571 | 5' | -59.6 | NC_004682.1 | + | 1564 | 0.71 | 0.226808 |
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Target: 5'- gGUCCUGgUCGAAGACa-UGGAGACgGg -3' miRNA: 3'- -CGGGACgAGCUUCUGggGCCUCUGgC- -5' |
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| 18571 | 5' | -59.6 | NC_004682.1 | + | 48535 | 0.72 | 0.215526 |
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Target: 5'- cGCCCUucgGCcCGGacGGACCuuGGGGGCCu -3' miRNA: 3'- -CGGGA---CGaGCU--UCUGGggCCUCUGGc -5' |
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| 18571 | 5' | -59.6 | NC_004682.1 | + | 27172 | 0.73 | 0.179771 |
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Target: 5'- gGCCCUGCUCGgcGACCCacuucgcGCCGg -3' miRNA: 3'- -CGGGACGAGCuuCUGGGgccuc--UGGC- -5' |
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| 18571 | 5' | -59.6 | NC_004682.1 | + | 42945 | 0.77 | 0.099449 |
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Target: 5'- cCUCUGCgaGggGAUCUCGGAGACCGg -3' miRNA: 3'- cGGGACGagCuuCUGGGGCCUCUGGC- -5' |
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| 18571 | 5' | -59.6 | NC_004682.1 | + | 50377 | 0.78 | 0.084033 |
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Target: 5'- gGCCCUggaggccGCUgGAuaccGGCCCUGGAGGCCGg -3' miRNA: 3'- -CGGGA-------CGAgCUu---CUGGGGCCUCUGGC- -5' |
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| 18571 | 5' | -59.6 | NC_004682.1 | + | 33839 | 1.09 | 0.00039 |
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Target: 5'- gGCCCUGCUCGAAGACCCCGGAGACCGc -3' miRNA: 3'- -CGGGACGAGCUUCUGGGGCCUCUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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