Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18693 | 3' | -59.1 | NC_004682.1 | + | 3566 | 0.66 | 0.506969 |
Target: 5'- aGGCGggggguuCACCGUCCGCucccggcccagcaGGCUGCAUg -3' miRNA: 3'- gCCGC-------GUGGCGGGCGuag----------CUGAUGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 83 | 0.66 | 0.486704 |
Target: 5'- aGGCucagaGCCGCCCucGCGggCGACUGgACa -3' miRNA: 3'- gCCGcg---UGGCGGG--CGUa-GCUGAUgUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 5885 | 0.66 | 0.486704 |
Target: 5'- uCGaGCGCGuuGCUCGCGaCGGCgucCACc -3' miRNA: 3'- -GC-CGCGUggCGGGCGUaGCUGau-GUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 28790 | 0.66 | 0.480699 |
Target: 5'- aCGGCGUcaucuucgucccgccGCCGCCgCgagugggcgGCAUCGGC-ACGCc -3' miRNA: 3'- -GCCGCG---------------UGGCGG-G---------CGUAGCUGaUGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 16898 | 0.66 | 0.476716 |
Target: 5'- uCGGCGCguucGgUGCCgGUGUCGGCgcugugGCGCu -3' miRNA: 3'- -GCCGCG----UgGCGGgCGUAGCUGa-----UGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 25567 | 0.66 | 0.476716 |
Target: 5'- uCGGCaGCACUGCCaccgGCugaggUGugUGCGCu -3' miRNA: 3'- -GCCG-CGUGGCGGg---CGua---GCugAUGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 44799 | 0.66 | 0.476716 |
Target: 5'- uGGC-CACCGgCgGCGUCGGCaUGCcCg -3' miRNA: 3'- gCCGcGUGGCgGgCGUAGCUG-AUGuG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 6017 | 0.66 | 0.466831 |
Target: 5'- uGGCcagccaGCGCCGCCCuugcCGUCGAgCUGCu- -3' miRNA: 3'- gCCG------CGUGGCGGGc---GUAGCU-GAUGug -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 42279 | 0.66 | 0.466831 |
Target: 5'- gCGGUGCAuCCGCa-GCA-CGGCUGC-Cg -3' miRNA: 3'- -GCCGCGU-GGCGggCGUaGCUGAUGuG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 3213 | 0.66 | 0.465848 |
Target: 5'- gCGGCugcuGCGCCGCCUGCGccagugaUCGACc---- -3' miRNA: 3'- -GCCG----CGUGGCGGGCGU-------AGCUGaugug -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 25995 | 0.67 | 0.457053 |
Target: 5'- aGGCGCaACCGCgCCGUgcGUCuGGCUGgGu -3' miRNA: 3'- gCCGCG-UGGCG-GGCG--UAG-CUGAUgUg -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 26335 | 0.67 | 0.457053 |
Target: 5'- gCGGUGUacgucuucgACCGCCUGUuccccuGUCGGC-GCGCa -3' miRNA: 3'- -GCCGCG---------UGGCGGGCG------UAGCUGaUGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 2021 | 0.67 | 0.457053 |
Target: 5'- aCGGCGCAcCCGUCCaggGUGUCGAggguCcACGCg -3' miRNA: 3'- -GCCGCGU-GGCGGG---CGUAGCU----GaUGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 4713 | 0.67 | 0.437833 |
Target: 5'- aGGUGCuggaucGCCGUgugCCGCAggCGcGCUACACg -3' miRNA: 3'- gCCGCG------UGGCG---GGCGUa-GC-UGAUGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 15063 | 0.67 | 0.428399 |
Target: 5'- cCGGCGCACCgacGCCUuguggGCGUgGAagccgACACg -3' miRNA: 3'- -GCCGCGUGG---CGGG-----CGUAgCUga---UGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 46400 | 0.67 | 0.428399 |
Target: 5'- gGGcCGCACgCGCUucgacaGCAaCGGCUACGCc -3' miRNA: 3'- gCC-GCGUG-GCGGg-----CGUaGCUGAUGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 9287 | 0.67 | 0.427462 |
Target: 5'- uGGCGCggAUgGCUCGCAucaccugUCGGgUGCGCg -3' miRNA: 3'- gCCGCG--UGgCGGGCGU-------AGCUgAUGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 10599 | 0.68 | 0.400833 |
Target: 5'- -cGCGCACUGCUCGUAgcgCGAcCUGgGCc -3' miRNA: 3'- gcCGCGUGGCGGGCGUa--GCU-GAUgUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 8307 | 0.68 | 0.391899 |
Target: 5'- uGGaCGcCACCGUCCGC-UCGAUguuCGCc -3' miRNA: 3'- gCC-GC-GUGGCGGGCGuAGCUGau-GUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 41527 | 0.68 | 0.383095 |
Target: 5'- uGGCGCggucgacgAgCGCCUGCGuggUCGGcCUGCACc -3' miRNA: 3'- gCCGCG--------UgGCGGGCGU---AGCU-GAUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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