Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18693 | 3' | -59.1 | NC_004682.1 | + | 39817 | 0.82 | 0.038055 |
Target: 5'- -aGCGCGCCGCCCGaugccUCGGCUGCGCu -3' miRNA: 3'- gcCGCGUGGCGGGCgu---AGCUGAUGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 13702 | 1.09 | 0.000356 |
Target: 5'- uCGGCGCACCGCCCGCAUCGACUACACg -3' miRNA: 3'- -GCCGCGUGGCGGGCGUAGCUGAUGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 5885 | 0.66 | 0.486704 |
Target: 5'- uCGaGCGCGuuGCUCGCGaCGGCgucCACc -3' miRNA: 3'- -GC-CGCGUggCGGGCGUaGCUGau-GUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 83 | 0.66 | 0.486704 |
Target: 5'- aGGCucagaGCCGCCCucGCGggCGACUGgACa -3' miRNA: 3'- gCCGcg---UGGCGGG--CGUa-GCUGAUgUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 16898 | 0.66 | 0.476716 |
Target: 5'- uCGGCGCguucGgUGCCgGUGUCGGCgcugugGCGCu -3' miRNA: 3'- -GCCGCG----UgGCGGgCGUAGCUGa-----UGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 25567 | 0.66 | 0.476716 |
Target: 5'- uCGGCaGCACUGCCaccgGCugaggUGugUGCGCu -3' miRNA: 3'- -GCCG-CGUGGCGGg---CGua---GCugAUGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 42279 | 0.66 | 0.466831 |
Target: 5'- gCGGUGCAuCCGCa-GCA-CGGCUGC-Cg -3' miRNA: 3'- -GCCGCGU-GGCGggCGUaGCUGAUGuG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 2021 | 0.67 | 0.457053 |
Target: 5'- aCGGCGCAcCCGUCCaggGUGUCGAggguCcACGCg -3' miRNA: 3'- -GCCGCGU-GGCGGG---CGUAGCU----GaUGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 26335 | 0.67 | 0.457053 |
Target: 5'- gCGGUGUacgucuucgACCGCCUGUuccccuGUCGGC-GCGCa -3' miRNA: 3'- -GCCGCG---------UGGCGGGCG------UAGCUGaUGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 4713 | 0.67 | 0.437833 |
Target: 5'- aGGUGCuggaucGCCGUgugCCGCAggCGcGCUACACg -3' miRNA: 3'- gCCGCG------UGGCG---GGCGUa-GC-UGAUGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 8307 | 0.68 | 0.391899 |
Target: 5'- uGGaCGcCACCGUCCGC-UCGAUguuCGCc -3' miRNA: 3'- gCC-GC-GUGGCGGGCGuAGCUGau-GUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 6153 | 0.68 | 0.363351 |
Target: 5'- aCGGCGgGCCG-CUGCGagucgacggcuauuUCGGCUACGa -3' miRNA: 3'- -GCCGCgUGGCgGGCGU--------------AGCUGAUGUg -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 28099 | 0.68 | 0.357484 |
Target: 5'- gGGCGUgGCUGCUCGCggCGGCgaGCGCg -3' miRNA: 3'- gCCGCG-UGGCGGGCGuaGCUGa-UGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 14590 | 0.69 | 0.341089 |
Target: 5'- uCGGCuacguguaCACCGCCCcgguggGCA-CGGCUGCGCc -3' miRNA: 3'- -GCCGc-------GUGGCGGG------CGUaGCUGAUGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 13852 | 0.69 | 0.332307 |
Target: 5'- aGGCcaaccugGCACagGCCCGCGcaUCGACgcgcugGCACa -3' miRNA: 3'- gCCG-------CGUGg-CGGGCGU--AGCUGa-----UGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 36867 | 0.7 | 0.288057 |
Target: 5'- aGGCGacgaucuuCGCCGCCCGCcugAUCGGCUugcuCAg -3' miRNA: 3'- gCCGC--------GUGGCGGGCG---UAGCUGAu---GUg -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 28298 | 0.72 | 0.216459 |
Target: 5'- gGGCGUuggccucggcauggACUGCCaCGCAUCGACccACGCc -3' miRNA: 3'- gCCGCG--------------UGGCGG-GCGUAGCUGa-UGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 35112 | 0.73 | 0.181615 |
Target: 5'- cCGGCGCacACCGCCgGCcaccaCGcACUGCGCg -3' miRNA: 3'- -GCCGCG--UGGCGGgCGua---GC-UGAUGUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 19965 | 0.78 | 0.075427 |
Target: 5'- gCGGC-CAUCGCCCGCAacgCGACUGcCGCg -3' miRNA: 3'- -GCCGcGUGGCGGGCGUa--GCUGAU-GUG- -5' |
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18693 | 3' | -59.1 | NC_004682.1 | + | 43980 | 0.68 | 0.365886 |
Target: 5'- aGGgGCACCGCUCGUAcugcUUGAgCUgagACACg -3' miRNA: 3'- gCCgCGUGGCGGGCGU----AGCU-GA---UGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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