Results 41 - 50 of 50 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18753 | 3' | -59.6 | NC_004683.1 | + | 24344 | 0.71 | 0.257441 |
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Target: 5'- -gCGCGGCguaGGACUCGGCGuaGGcgCGGg -3' miRNA: 3'- gaGCGCCGg--CUUGAGCCGC--CCuaGCU- -5' |
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| 18753 | 3' | -59.6 | NC_004683.1 | + | 565 | 0.72 | 0.245013 |
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Target: 5'- --gGUGGCCGAccugugcgACUUGGCGGGGUUc- -3' miRNA: 3'- gagCGCCGGCU--------UGAGCCGCCCUAGcu -5' |
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| 18753 | 3' | -59.6 | NC_004683.1 | + | 53611 | 0.72 | 0.245013 |
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Target: 5'- uUCGCcGCCGAGCUgCGGCGcGAUCa- -3' miRNA: 3'- gAGCGcCGGCUUGA-GCCGCcCUAGcu -5' |
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| 18753 | 3' | -59.6 | NC_004683.1 | + | 26559 | 0.72 | 0.216118 |
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Target: 5'- cCUCGuCGGCguauCGGGCUCGGCGGGcggccuggAUCGc -3' miRNA: 3'- -GAGC-GCCG----GCUUGAGCCGCCC--------UAGCu -5' |
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| 18753 | 3' | -59.6 | NC_004683.1 | + | 2759 | 0.73 | 0.210701 |
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Target: 5'- -cCGCGG-CGAGCUCGGCGGcGGccaacUCGGu -3' miRNA: 3'- gaGCGCCgGCUUGAGCCGCC-CU-----AGCU- -5' |
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| 18753 | 3' | -59.6 | NC_004683.1 | + | 49091 | 0.74 | 0.180609 |
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Target: 5'- uUCcCGGUCGGGCUugagggCGGCGGGAUCGc -3' miRNA: 3'- gAGcGCCGGCUUGA------GCCGCCCUAGCu -5' |
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| 18753 | 3' | -59.6 | NC_004683.1 | + | 41007 | 0.74 | 0.175979 |
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Target: 5'- -gCGCGGUC-AGCUCGGCGcccGGGUCGGa -3' miRNA: 3'- gaGCGCCGGcUUGAGCCGC---CCUAGCU- -5' |
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| 18753 | 3' | -59.6 | NC_004683.1 | + | 20180 | 0.74 | 0.171456 |
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Target: 5'- -cCGCGGCCucaacaccagGAACgacCGGCGGGGUCa- -3' miRNA: 3'- gaGCGCCGG----------CUUGa--GCCGCCCUAGcu -5' |
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| 18753 | 3' | -59.6 | NC_004683.1 | + | 10124 | 0.77 | 0.115125 |
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Target: 5'- -cCGCGGCCGAacgGCUCGGCGGcg-UGAg -3' miRNA: 3'- gaGCGCCGGCU---UGAGCCGCCcuaGCU- -5' |
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| 18753 | 3' | -59.6 | NC_004683.1 | + | 1437 | 1.08 | 0.000525 |
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Target: 5'- gCUCGCGGCCGAACUCGGCGGGAUCGAg -3' miRNA: 3'- -GAGCGCCGGCUUGAGCCGCCCUAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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