Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18759 | 3' | -53.5 | NC_004683.1 | + | 48955 | 0.71 | 0.567447 |
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Target: 5'- aUCGAGAACCGGAcccGCaACAUcGCCGg-- -3' miRNA: 3'- -GGCUCUUGGCCU---UGcUGUA-CGGCagc -5' |
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| 18759 | 3' | -53.5 | NC_004683.1 | + | 49443 | 0.67 | 0.827199 |
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Target: 5'- uCCGGGGcagguGCaGGGGC-ACcgGCCGUCGu -3' miRNA: 3'- -GGCUCU-----UGgCCUUGcUGuaCGGCAGC- -5' |
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| 18759 | 3' | -53.5 | NC_004683.1 | + | 51015 | 0.69 | 0.675837 |
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Target: 5'- cCCGAGGGCUGGAugcccgACGACGagacgaucGCCG-CGa -3' miRNA: 3'- -GGCUCUUGGCCU------UGCUGUa-------CGGCaGC- -5' |
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| 18759 | 3' | -53.5 | NC_004683.1 | + | 51167 | 0.66 | 0.844733 |
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Target: 5'- gCG-GAACUGGAucCGGCGcGCCGcCGa -3' miRNA: 3'- gGCuCUUGGCCUu-GCUGUaCGGCaGC- -5' |
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| 18759 | 3' | -53.5 | NC_004683.1 | + | 51228 | 0.68 | 0.749843 |
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Target: 5'- gCGucGAACgGGcuCGGCAaGCCGUCGc -3' miRNA: 3'- gGCu-CUUGgCCuuGCUGUaCGGCAGC- -5' |
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| 18759 | 3' | -53.5 | NC_004683.1 | + | 51325 | 0.67 | 0.799419 |
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Target: 5'- gCCGAGGGCCuucuGCGACGgcuUGCCGa-- -3' miRNA: 3'- -GGCUCUUGGccu-UGCUGU---ACGGCagc -5' |
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| 18759 | 3' | -53.5 | NC_004683.1 | + | 51875 | 0.66 | 0.869379 |
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Target: 5'- uUGAGGuuGCgGGcuGugGACAUGCCGagGg -3' miRNA: 3'- gGCUCU--UGgCC--UugCUGUACGGCagC- -5' |
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| 18759 | 3' | -53.5 | NC_004683.1 | + | 53101 | 0.67 | 0.799419 |
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Target: 5'- cCCGAGccaucGGCCGGGGCGuuc-GUCGUUGg -3' miRNA: 3'- -GGCUC-----UUGGCCUUGCuguaCGGCAGC- -5' |
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| 18759 | 3' | -53.5 | NC_004683.1 | + | 56861 | 0.66 | 0.869379 |
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Target: 5'- cUCGGGAcCuCGGuAGCGGCugcgGUCGUCGu -3' miRNA: 3'- -GGCUCUuG-GCC-UUGCUGua--CGGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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