Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18759 | 5' | -59.8 | NC_004683.1 | + | 2369 | 0.68 | 0.396477 |
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Target: 5'- cGGUGGUGGCGUCccgCGGauccagcagacACUGGccGGCCc -3' miRNA: 3'- -CCGCUACCGCAGa--GCU-----------UGGCC--CCGGu -5' |
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| 18759 | 5' | -59.8 | NC_004683.1 | + | 431 | 0.67 | 0.431574 |
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Target: 5'- aGGCGGUGGCuggcgcggcgGUCgaggCGGAuCCGGuggacgaguugaaGGCCAg -3' miRNA: 3'- -CCGCUACCG----------CAGa---GCUU-GGCC-------------CCGGU- -5' |
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| 18759 | 5' | -59.8 | NC_004683.1 | + | 2132 | 0.67 | 0.469388 |
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Target: 5'- uGGUGGUGGCGgg-CGuGGCCGugcuggcGGGCCu -3' miRNA: 3'- -CCGCUACCGCagaGC-UUGGC-------CCCGGu -5' |
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| 18759 | 5' | -59.8 | NC_004683.1 | + | 31798 | 0.66 | 0.498828 |
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Target: 5'- -aUGGUGGUGUCUCcgauguaGAACCGcccuaGGCCAa -3' miRNA: 3'- ccGCUACCGCAGAG-------CUUGGCc----CCGGU- -5' |
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| 18759 | 5' | -59.8 | NC_004683.1 | + | 6959 | 0.77 | 0.096018 |
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Target: 5'- gGGCGGUGGUGcagCUCGucgGCCGGcGGCCc -3' miRNA: 3'- -CCGCUACCGCa--GAGCu--UGGCC-CCGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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