Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18763 | 5' | -52.5 | NC_004683.1 | + | 22995 | 0.66 | 0.887867 |
Target: 5'- cCGUCGuUGAUCACcaGCGACACc-- -3' miRNA: 3'- aGUAGCuACUAGUGccCGCUGUGcac -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 47151 | 0.66 | 0.872525 |
Target: 5'- aUCcUCGAcgucGAUCGuCGGGUGGC-CGUGc -3' miRNA: 3'- -AGuAGCUa---CUAGU-GCCCGCUGuGCAC- -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 52143 | 0.66 | 0.872525 |
Target: 5'- gCGUCGAcGAUCAgccaGGGCGcgucggGCACGg- -3' miRNA: 3'- aGUAGCUaCUAGUg---CCCGC------UGUGCac -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 23472 | 0.66 | 0.867722 |
Target: 5'- cUCGUCGGUGccgcCGCGGGCguuguagagcgacucGACguagGCGUGg -3' miRNA: 3'- -AGUAGCUACua--GUGCCCG---------------CUG----UGCAC- -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 6460 | 0.67 | 0.847628 |
Target: 5'- gUCGUCGAacaGGUCGCcgGGGCGcaccacgcgggGCACGUu -3' miRNA: 3'- -AGUAGCUa--CUAGUG--CCCGC-----------UGUGCAc -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 39819 | 0.67 | 0.838858 |
Target: 5'- -gGUCGAUGAgUugGGGUc-CGCGUGa -3' miRNA: 3'- agUAGCUACUaGugCCCGcuGUGCAC- -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 22372 | 0.67 | 0.838858 |
Target: 5'- uUCAUUugcGAUCAUGGGCGAuucCACGg- -3' miRNA: 3'- -AGUAGcuaCUAGUGCCCGCU---GUGCac -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 21779 | 0.67 | 0.836183 |
Target: 5'- cCAcCGAUGGUuggucggguacggcCACGGGCGGcgUugGUGg -3' miRNA: 3'- aGUaGCUACUA--------------GUGCCCGCU--GugCAC- -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 28430 | 0.67 | 0.820662 |
Target: 5'- gUCAUCGAUGccgagcuGCGGGCGcuCGCGg- -3' miRNA: 3'- -AGUAGCUACuag----UGCCCGCu-GUGCac -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 3967 | 0.67 | 0.811256 |
Target: 5'- -gAUCGAgccGAUCAUgGGGUGGCGgcCGUGg -3' miRNA: 3'- agUAGCUa--CUAGUG-CCCGCUGU--GCAC- -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 42724 | 0.67 | 0.811256 |
Target: 5'- cUCAUCGgcGGccgccgaucucgUCGCGGGCGucguCGCGg- -3' miRNA: 3'- -AGUAGCuaCU------------AGUGCCCGCu---GUGCac -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 34757 | 0.67 | 0.810305 |
Target: 5'- aUCAgCGAaggaggaacuuguUGGUCACGaGCGugACGUGc -3' miRNA: 3'- -AGUaGCU-------------ACUAGUGCcCGCugUGCAC- -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 46785 | 0.68 | 0.801659 |
Target: 5'- uUCAUCGcgGAgUUGCGcGGCGGCGgGUc -3' miRNA: 3'- -AGUAGCuaCU-AGUGC-CCGCUGUgCAc -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 42517 | 0.68 | 0.791881 |
Target: 5'- gUCGUCaGGguuggGAUCGCGGucGgGAUACGUGa -3' miRNA: 3'- -AGUAG-CUa----CUAGUGCC--CgCUGUGCAC- -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 41403 | 0.68 | 0.771827 |
Target: 5'- aUCGUCGAcgccgucagccUGG--GCGGGUGGCGCGg- -3' miRNA: 3'- -AGUAGCU-----------ACUagUGCCCGCUGUGCac -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 15099 | 0.68 | 0.761574 |
Target: 5'- gCAUCGGUGcgCaagaucgccGCGGGCGACAa--- -3' miRNA: 3'- aGUAGCUACuaG---------UGCCCGCUGUgcac -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 21548 | 0.68 | 0.751186 |
Target: 5'- aCAUCGAcgUGAUCcuGCuGGGCGGCgGCGgUGg -3' miRNA: 3'- aGUAGCU--ACUAG--UG-CCCGCUG-UGC-AC- -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 16591 | 0.68 | 0.751186 |
Target: 5'- gCGUCGAUGu--GCGGGuCGACcUGUGg -3' miRNA: 3'- aGUAGCUACuagUGCCC-GCUGuGCAC- -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 45472 | 0.68 | 0.751186 |
Target: 5'- -gGUUGgcGGUCAgGGGUGGC-CGUGa -3' miRNA: 3'- agUAGCuaCUAGUgCCCGCUGuGCAC- -5' |
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18763 | 5' | -52.5 | NC_004683.1 | + | 21688 | 0.69 | 0.730052 |
Target: 5'- aUCAUCaccggGGUGGUCgguGCGGGUGGCGC-UGg -3' miRNA: 3'- -AGUAG-----CUACUAG---UGCCCGCUGUGcAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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