Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18763 | 5' | -52.5 | NC_004683.1 | + | 14308 | 0.69 | 0.708518 |
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Target: 5'- uUCAUCGAccucGAU-ACGGGCcGCugGUGg -3' miRNA: 3'- -AGUAGCUa---CUAgUGCCCGcUGugCAC- -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 40840 | 0.7 | 0.653549 |
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Target: 5'- cCAUCGAagugccgagugUGcAUCGCGGGCGAgUACGg- -3' miRNA: 3'- aGUAGCU-----------AC-UAGUGCCCGCU-GUGCac -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 52710 | 0.7 | 0.642451 |
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Target: 5'- cUCGUCGGUGAaCACGuGCuGGCACGg- -3' miRNA: 3'- -AGUAGCUACUaGUGCcCG-CUGUGCac -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 50030 | 0.71 | 0.587017 |
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Target: 5'- uUCGUCGuUGA-CGCGGcguGCGACGCGUu -3' miRNA: 3'- -AGUAGCuACUaGUGCC---CGCUGUGCAc -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 6245 | 0.73 | 0.500704 |
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Target: 5'- gUCAUCGAggGggCGCGGGCGAucccCACGc- -3' miRNA: 3'- -AGUAGCUa-CuaGUGCCCGCU----GUGCac -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 2123 | 0.73 | 0.469747 |
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Target: 5'- cCAUCGcccugGUGGUgGCGGGCGugGcCGUGc -3' miRNA: 3'- aGUAGC-----UACUAgUGCCCGCugU-GCAC- -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 6715 | 0.78 | 0.274292 |
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Target: 5'- aUCAUCGAUGAcgUCAuggaagucgacggccCGGGCGGcCGCGUGc -3' miRNA: 3'- -AGUAGCUACU--AGU---------------GCCCGCU-GUGCAC- -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 6782 | 1.08 | 0.002448 |
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Target: 5'- gUCAUCGAUGAUCACGGGCGACACGUGc -3' miRNA: 3'- -AGUAGCUACUAGUGCCCGCUGUGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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