Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18763 | 5' | -52.5 | NC_004683.1 | + | 21779 | 0.67 | 0.836183 |
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Target: 5'- cCAcCGAUGGUuggucggguacggcCACGGGCGGcgUugGUGg -3' miRNA: 3'- aGUaGCUACUA--------------GUGCCCGCU--GugCAC- -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 39819 | 0.67 | 0.838858 |
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Target: 5'- -gGUCGAUGAgUugGGGUc-CGCGUGa -3' miRNA: 3'- agUAGCUACUaGugCCCGcuGUGCAC- -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 22372 | 0.67 | 0.838858 |
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Target: 5'- uUCAUUugcGAUCAUGGGCGAuucCACGg- -3' miRNA: 3'- -AGUAGcuaCUAGUGCCCGCU---GUGCac -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 6460 | 0.67 | 0.847628 |
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Target: 5'- gUCGUCGAacaGGUCGCcgGGGCGcaccacgcgggGCACGUu -3' miRNA: 3'- -AGUAGCUa--CUAGUG--CCCGC-----------UGUGCAc -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 23472 | 0.66 | 0.867722 |
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Target: 5'- cUCGUCGGUGccgcCGCGGGCguuguagagcgacucGACguagGCGUGg -3' miRNA: 3'- -AGUAGCUACua--GUGCCCG---------------CUG----UGCAC- -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 52143 | 0.66 | 0.872525 |
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Target: 5'- gCGUCGAcGAUCAgccaGGGCGcgucggGCACGg- -3' miRNA: 3'- aGUAGCUaCUAGUg---CCCGC------UGUGCac -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 47151 | 0.66 | 0.872525 |
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Target: 5'- aUCcUCGAcgucGAUCGuCGGGUGGC-CGUGc -3' miRNA: 3'- -AGuAGCUa---CUAGU-GCCCGCUGuGCAC- -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 22995 | 0.66 | 0.887867 |
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Target: 5'- cCGUCGuUGAUCACcaGCGACACc-- -3' miRNA: 3'- aGUAGCuACUAGUGccCGCUGUGcac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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