Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18763 | 5' | -52.5 | NC_004683.1 | + | 34757 | 0.67 | 0.810305 |
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Target: 5'- aUCAgCGAaggaggaacuuguUGGUCACGaGCGugACGUGc -3' miRNA: 3'- -AGUaGCU-------------ACUAGUGCcCGCugUGCAC- -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 42517 | 0.68 | 0.791881 |
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Target: 5'- gUCGUCaGGguuggGAUCGCGGucGgGAUACGUGa -3' miRNA: 3'- -AGUAG-CUa----CUAGUGCC--CgCUGUGCAC- -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 41403 | 0.68 | 0.771827 |
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Target: 5'- aUCGUCGAcgccgucagccUGG--GCGGGUGGCGCGg- -3' miRNA: 3'- -AGUAGCU-----------ACUagUGCCCGCUGUGCac -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 45472 | 0.68 | 0.751186 |
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Target: 5'- -gGUUGgcGGUCAgGGGUGGC-CGUGa -3' miRNA: 3'- agUAGCuaCUAGUgCCCGCUGuGCAC- -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 52710 | 0.7 | 0.642451 |
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Target: 5'- cUCGUCGGUGAaCACGuGCuGGCACGg- -3' miRNA: 3'- -AGUAGCUACUaGUGCcCG-CUGUGCac -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 50030 | 0.71 | 0.587017 |
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Target: 5'- uUCGUCGuUGA-CGCGGcguGCGACGCGUu -3' miRNA: 3'- -AGUAGCuACUaGUGCC---CGCUGUGCAc -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 6782 | 1.08 | 0.002448 |
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Target: 5'- gUCAUCGAUGAUCACGGGCGACACGUGc -3' miRNA: 3'- -AGUAGCUACUAGUGCCCGCUGUGCAC- -5' |
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| 18763 | 5' | -52.5 | NC_004683.1 | + | 22995 | 0.66 | 0.887867 |
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Target: 5'- cCGUCGuUGAUCACcaGCGACACc-- -3' miRNA: 3'- aGUAGCuACUAGUGccCGCUGUGcac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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