Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18766 | 3' | -55.4 | NC_004683.1 | + | 18310 | 0.66 | 0.756635 |
Target: 5'- cCGaCAaUCUUGCCCGUG--GGCGAGu- -3' miRNA: 3'- -GC-GUaAGAACGGGCGCagCCGCUUgc -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 30049 | 0.66 | 0.746387 |
Target: 5'- uGCA--CUUGUCCGCaUCGGaucuGGACGg -3' miRNA: 3'- gCGUaaGAACGGGCGcAGCCg---CUUGC- -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 52609 | 0.67 | 0.693666 |
Target: 5'- gGCAUUcCUUGgCCGCcucGUCGGCc-GCGa -3' miRNA: 3'- gCGUAA-GAACgGGCG---CAGCCGcuUGC- -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 1955 | 0.67 | 0.682904 |
Target: 5'- gCGCA-UCUUGCCUGCGgucaccgaaUCGGCcagaggcuucGAGCc -3' miRNA: 3'- -GCGUaAGAACGGGCGC---------AGCCG----------CUUGc -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 33929 | 0.67 | 0.682904 |
Target: 5'- cCGUcUUCgcgGgCCGCGUCGGCuGGAuCGg -3' miRNA: 3'- -GCGuAAGaa-CgGGCGCAGCCG-CUU-GC- -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 31198 | 0.67 | 0.682904 |
Target: 5'- gCGCGUUCUcacgaacacacUGCCCccgcGgGUgGGCaGAGCGa -3' miRNA: 3'- -GCGUAAGA-----------ACGGG----CgCAgCCG-CUUGC- -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 52726 | 0.67 | 0.672094 |
Target: 5'- uGCGUUCggcGCguuCCuCGUCGGUGAACa -3' miRNA: 3'- gCGUAAGaa-CG---GGcGCAGCCGCUUGc -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 49063 | 0.67 | 0.672094 |
Target: 5'- uCGCGgcgg-GCCCGCGguacuuucCGGCGAuguuGCGg -3' miRNA: 3'- -GCGUaagaaCGGGCGCa-------GCCGCU----UGC- -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 7840 | 0.67 | 0.661247 |
Target: 5'- uGC-UUCUUGUCUGCGcCGGUgccGAACu -3' miRNA: 3'- gCGuAAGAACGGGCGCaGCCG---CUUGc -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 21934 | 0.67 | 0.650373 |
Target: 5'- gGCAaUCc-GCCCgGCGgUGGCGGGCGg -3' miRNA: 3'- gCGUaAGaaCGGG-CGCaGCCGCUUGC- -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 39516 | 0.67 | 0.650373 |
Target: 5'- gGCGaggUCcUGgCCGCGagGGCGAAUGu -3' miRNA: 3'- gCGUa--AGaACgGGCGCagCCGCUUGC- -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 5827 | 0.67 | 0.650373 |
Target: 5'- gGCGgcggUGCCCGCGagcgCGGUGA-CGa -3' miRNA: 3'- gCGUaagaACGGGCGCa---GCCGCUuGC- -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 27160 | 0.68 | 0.639483 |
Target: 5'- uGCGcUCgUUGCCCGacuuGUCGGUGuaGACGa -3' miRNA: 3'- gCGUaAG-AACGGGCg---CAGCCGC--UUGC- -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 34011 | 0.68 | 0.617692 |
Target: 5'- gGCGUUCa-GCuuGCGUCaGCGuGCGa -3' miRNA: 3'- gCGUAAGaaCGggCGCAGcCGCuUGC- -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 13681 | 0.68 | 0.611162 |
Target: 5'- gGCGagCUgGCCCGCauagccgaucgcgcgGUUGGCGAGCu -3' miRNA: 3'- gCGUaaGAaCGGGCG---------------CAGCCGCUUGc -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 7668 | 0.68 | 0.603551 |
Target: 5'- aGuCGUUgaUGCCCGCGUCGaccucggccgaguuGCGAAUa -3' miRNA: 3'- gC-GUAAgaACGGGCGCAGC--------------CGCUUGc -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 12218 | 0.7 | 0.521221 |
Target: 5'- gGCcgaaUCggcccUGCCUGCGUCGGCGAccuGCu -3' miRNA: 3'- gCGua--AGa----ACGGGCGCAGCCGCU---UGc -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 41286 | 0.71 | 0.440729 |
Target: 5'- gGCGUccUCggcgGCcuucgCCGCGUCGGCGggUGu -3' miRNA: 3'- gCGUA--AGaa--CG-----GGCGCAGCCGCuuGC- -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 3370 | 0.72 | 0.39415 |
Target: 5'- uGCcgUCg-GCCagGCGUCGGCGGGCc -3' miRNA: 3'- gCGuaAGaaCGGg-CGCAGCCGCUUGc -5' |
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18766 | 3' | -55.4 | NC_004683.1 | + | 56318 | 0.72 | 0.367776 |
Target: 5'- gCGCGUUCUcgaugucggguUGCCaGCGUCGGgGAuacugGCGg -3' miRNA: 3'- -GCGUAAGA-----------ACGGgCGCAGCCgCU-----UGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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