Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18766 | 3' | -55.4 | NC_004683.1 | + | 7668 | 0.68 | 0.603551 |
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Target: 5'- aGuCGUUgaUGCCCGCGUCGaccucggccgaguuGCGAAUa -3' miRNA: 3'- gC-GUAAgaACGGGCGCAGC--------------CGCUUGc -5' |
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| 18766 | 3' | -55.4 | NC_004683.1 | + | 5827 | 0.67 | 0.650373 |
|
Target: 5'- gGCGgcggUGCCCGCGagcgCGGUGA-CGa -3' miRNA: 3'- gCGUaagaACGGGCGCa---GCCGCUuGC- -5' |
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| 18766 | 3' | -55.4 | NC_004683.1 | + | 3569 | 0.73 | 0.326584 |
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Target: 5'- aGCGUucaacUCUUGCcgcccggcguguCCGCGUCGGCgucGAGCGa -3' miRNA: 3'- gCGUA-----AGAACG------------GGCGCAGCCG---CUUGC- -5' |
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| 18766 | 3' | -55.4 | NC_004683.1 | + | 3370 | 0.72 | 0.39415 |
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Target: 5'- uGCcgUCg-GCCagGCGUCGGCGGGCc -3' miRNA: 3'- gCGuaAGaaCGGg-CGCAGCCGCUUGc -5' |
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| 18766 | 3' | -55.4 | NC_004683.1 | + | 1955 | 0.67 | 0.682904 |
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Target: 5'- gCGCA-UCUUGCCUGCGgucaccgaaUCGGCcagaggcuucGAGCc -3' miRNA: 3'- -GCGUaAGAACGGGCGC---------AGCCG----------CUUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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