Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18769 | 5' | -58.5 | NC_004683.1 | + | 13102 | 0.69 | 0.387047 |
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Target: 5'- ---aCCCGGAaGUCGCCgGGGccaccgGCGCc -3' miRNA: 3'- cgugGGGCCUaCAGCGGaCCUa-----CGCG- -5' |
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| 18769 | 5' | -58.5 | NC_004683.1 | + | 39701 | 0.69 | 0.369866 |
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Target: 5'- cCACCCCGGcgG-CGCCcaccccGGAacggGCGCc -3' miRNA: 3'- cGUGGGGCCuaCaGCGGa-----CCUa---CGCG- -5' |
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| 18769 | 5' | -58.5 | NC_004683.1 | + | 1167 | 0.71 | 0.284279 |
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Target: 5'- gGCGCCCCGuaag-CGCCUGGGcuaccuggaguucUGUGCa -3' miRNA: 3'- -CGUGGGGCcuacaGCGGACCU-------------ACGCG- -5' |
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| 18769 | 5' | -58.5 | NC_004683.1 | + | 19091 | 0.73 | 0.23344 |
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Target: 5'- gGCGCgCCUGGAUcuacccCGCUgGGAUGCGCg -3' miRNA: 3'- -CGUG-GGGCCUAca----GCGGaCCUACGCG- -5' |
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| 18769 | 5' | -58.5 | NC_004683.1 | + | 20501 | 0.73 | 0.215124 |
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Target: 5'- uGCACCgCCGG-UGcCGCCguuggccgccccGGGUGCGCc -3' miRNA: 3'- -CGUGG-GGCCuACaGCGGa-----------CCUACGCG- -5' |
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| 18769 | 5' | -58.5 | NC_004683.1 | + | 49313 | 0.74 | 0.194989 |
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Target: 5'- gGCGCCCCGGAcgugugGUUGCUggccgaucgGGAUaggGCGCg -3' miRNA: 3'- -CGUGGGGCCUa-----CAGCGGa--------CCUA---CGCG- -5' |
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| 18769 | 5' | -58.5 | NC_004683.1 | + | 11195 | 1.13 | 0.000287 |
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Target: 5'- aGCACCCCGGAUGUCGCCUGGAUGCGCu -3' miRNA: 3'- -CGUGGGGCCUACAGCGGACCUACGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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