Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18780 | 5' | -57.8 | NC_004683.1 | + | 40204 | 0.67 | 0.508913 |
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Target: 5'- cAGAUUGGCGAUG-GucGCgGCCGCGu -3' miRNA: 3'- aUCUAGUCGCUGUaCucCGgCGGCGC- -5' |
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| 18780 | 5' | -57.8 | NC_004683.1 | + | 3734 | 0.67 | 0.488394 |
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Target: 5'- ----cCAGCGGCGgccGGGCCGCaCGCu -3' miRNA: 3'- aucuaGUCGCUGUac-UCCGGCG-GCGc -5' |
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| 18780 | 5' | -57.8 | NC_004683.1 | + | 56993 | 0.68 | 0.458371 |
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Target: 5'- cAGGUCAGaGGCucgcgGGGuGCCGCUGCa -3' miRNA: 3'- aUCUAGUCgCUGua---CUC-CGGCGGCGc -5' |
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| 18780 | 5' | -57.8 | NC_004683.1 | + | 6350 | 0.71 | 0.302068 |
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Target: 5'- gGGGUCAGCcaccgagggGGCGUGGGGaUCGcCCGCGc -3' miRNA: 3'- aUCUAGUCG---------CUGUACUCC-GGC-GGCGC- -5' |
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| 18780 | 5' | -57.8 | NC_004683.1 | + | 23813 | 0.72 | 0.247013 |
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Target: 5'- gGGGUCGGCG-CcgGGGugguGCCGCCGCc -3' miRNA: 3'- aUCUAGUCGCuGuaCUC----CGGCGGCGc -5' |
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| 18780 | 5' | -57.8 | NC_004683.1 | + | 35028 | 0.73 | 0.234615 |
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Target: 5'- ---uUCAGCuGACGgccGAGGCCGUCGCa -3' miRNA: 3'- aucuAGUCG-CUGUa--CUCCGGCGGCGc -5' |
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| 18780 | 5' | -57.8 | NC_004683.1 | + | 17443 | 1.08 | 0.000651 |
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Target: 5'- gUAGAUCAGCGACAUGAGGCCGCCGCGg -3' miRNA: 3'- -AUCUAGUCGCUGUACUCCGGCGGCGC- -5' |
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| 18780 | 5' | -57.8 | NC_004683.1 | + | 16067 | 0.66 | 0.583188 |
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Target: 5'- cGGGUCGGUGcccgaugccucCAUGAGGUCGgCGCc -3' miRNA: 3'- aUCUAGUCGCu----------GUACUCCGGCgGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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