Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18987 | 3' | -56.7 | NC_004684.1 | + | 10387 | 0.72 | 0.352388 |
Target: 5'- aUCGgugCgGGCGACCUCUggcgcgacaccgGCGUCGCUGg- -3' miRNA: 3'- -AGCa--G-UCGCUGGAGG------------UGCAGCGACag -5' |
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18987 | 3' | -56.7 | NC_004684.1 | + | 34970 | 0.73 | 0.313105 |
Target: 5'- gUCGUUGGCcaaGGCCUUCACGUCGCg--- -3' miRNA: 3'- -AGCAGUCG---CUGGAGGUGCAGCGacag -5' |
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18987 | 3' | -56.7 | NC_004684.1 | + | 65975 | 0.73 | 0.313105 |
Target: 5'- cUUGgccCGGCaGGCCUCCAgGUCGCUGg- -3' miRNA: 3'- -AGCa--GUCG-CUGGAGGUgCAGCGACag -5' |
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18987 | 3' | -56.7 | NC_004684.1 | + | 33645 | 0.84 | 0.059836 |
Target: 5'- cUCGUCAGCGACC-CCucgccgggcaugGCGUCGCUGUg -3' miRNA: 3'- -AGCAGUCGCUGGaGG------------UGCAGCGACAg -5' |
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18987 | 3' | -56.7 | NC_004684.1 | + | 7865 | 1.11 | 0.000758 |
Target: 5'- aUCGUCAGCGACCUCCACGUCGCUGUCg -3' miRNA: 3'- -AGCAGUCGCUGGAGGUGCAGCGACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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