Results 41 - 50 of 50 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18997 | 3' | -55.9 | NC_004684.1 | + | 61179 | 0.72 | 0.424317 |
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Target: 5'- cGUCGaCCGGuGG-CGGCGUUGCGgaGACAa -3' miRNA: 3'- -CAGC-GGCC-CUaGCUGCAACGUg-CUGU- -5' |
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| 18997 | 3' | -55.9 | NC_004684.1 | + | 49474 | 0.72 | 0.415091 |
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Target: 5'- uUCGCCGGGGUUGuCGguccagaugUGCAgCGGCGc -3' miRNA: 3'- cAGCGGCCCUAGCuGCa--------ACGU-GCUGU- -5' |
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| 18997 | 3' | -55.9 | NC_004684.1 | + | 40717 | 0.72 | 0.405989 |
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Target: 5'- gGUgGCCGGGA-CGACca-GCGCGGCGg -3' miRNA: 3'- -CAgCGGCCCUaGCUGcaaCGUGCUGU- -5' |
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| 18997 | 3' | -55.9 | NC_004684.1 | + | 63185 | 0.72 | 0.388169 |
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Target: 5'- -cCGCCGGG-UCGcCGUaauguagugcuUGCACGGCGg -3' miRNA: 3'- caGCGGCCCuAGCuGCA-----------ACGUGCUGU- -5' |
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| 18997 | 3' | -55.9 | NC_004684.1 | + | 53332 | 0.72 | 0.382923 |
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Target: 5'- -gCGCCGGGAUcaccuacccgugccaCGugGUggUGCugGGCAc -3' miRNA: 3'- caGCGGCCCUA---------------GCugCA--ACGugCUGU- -5' |
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| 18997 | 3' | -55.9 | NC_004684.1 | + | 50609 | 0.72 | 0.379452 |
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Target: 5'- gGUCGgCGGGucAUCGGCGUgGC-CGACAc -3' miRNA: 3'- -CAGCgGCCC--UAGCUGCAaCGuGCUGU- -5' |
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| 18997 | 3' | -55.9 | NC_004684.1 | + | 2391 | 0.73 | 0.370868 |
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Target: 5'- uUCGCCGGGugCGGCG-UGCACGuCGu -3' miRNA: 3'- cAGCGGCCCuaGCUGCaACGUGCuGU- -5' |
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| 18997 | 3' | -55.9 | NC_004684.1 | + | 35882 | 0.76 | 0.245744 |
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Target: 5'- cGUCGCCGGGGacugcgccaCGAUGgaGCGCGGCGc -3' miRNA: 3'- -CAGCGGCCCUa--------GCUGCaaCGUGCUGU- -5' |
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| 18997 | 3' | -55.9 | NC_004684.1 | + | 27126 | 0.8 | 0.123616 |
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Target: 5'- cGUUGCCGGGAcgcugaUCGACG-UGCugGGCGa -3' miRNA: 3'- -CAGCGGCCCU------AGCUGCaACGugCUGU- -5' |
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| 18997 | 3' | -55.9 | NC_004684.1 | + | 14339 | 1.08 | 0.00151 |
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Target: 5'- uGUCGCCGGGAUCGACGUUGCACGACAc -3' miRNA: 3'- -CAGCGGCCCUAGCUGCAACGUGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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