Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19044 | 3' | -55.2 | NC_004684.1 | + | 67194 | 0.67 | 0.738109 |
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Target: 5'- cGGGUCAcGGUCu-CGCCGUUcggcaggucaaCGGUGAa -3' miRNA: 3'- -CCUAGU-CCAGcuGCGGCAAc----------GCCACU- -5' |
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| 19044 | 3' | -55.2 | NC_004684.1 | + | 38493 | 0.67 | 0.747279 |
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Target: 5'- cGAUCAgcggcGGUCGccacggcGCGCCGUU-CGGUGu -3' miRNA: 3'- cCUAGU-----CCAGC-------UGCGGCAAcGCCACu -5' |
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| 19044 | 3' | -55.2 | NC_004684.1 | + | 42166 | 0.67 | 0.748293 |
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Target: 5'- ----uGGGUCGagaGCGCCGcacugcGCGGUGAg -3' miRNA: 3'- ccuagUCCAGC---UGCGGCaa----CGCCACU- -5' |
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| 19044 | 3' | -55.2 | NC_004684.1 | + | 55405 | 0.67 | 0.748293 |
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Target: 5'- gGGGUCAugccGUCGGugcCGCCGgucGCGGUGu -3' miRNA: 3'- -CCUAGUc---CAGCU---GCGGCaa-CGCCACu -5' |
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| 19044 | 3' | -55.2 | NC_004684.1 | + | 45763 | 0.66 | 0.787801 |
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Target: 5'- aGGuUCuuGGUC-ACGCCGgUGCGGUa- -3' miRNA: 3'- -CCuAGu-CCAGcUGCGGCaACGCCAcu -5' |
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| 19044 | 3' | -55.2 | NC_004684.1 | + | 23710 | 0.66 | 0.797319 |
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Target: 5'- cGGuGUCGGaccUCGACGCCG-UGUGGgcUGAg -3' miRNA: 3'- -CC-UAGUCc--AGCUGCGGCaACGCC--ACU- -5' |
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| 19044 | 3' | -55.2 | NC_004684.1 | + | 34848 | 0.66 | 0.805745 |
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Target: 5'- ---cCAGGUcaucgccCGGCGCgaaCGUUGUGGUGGc -3' miRNA: 3'- ccuaGUCCA-------GCUGCG---GCAACGCCACU- -5' |
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| 19044 | 3' | -55.2 | NC_004684.1 | + | 57535 | 0.66 | 0.815853 |
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Target: 5'- gGGAUCcGG-CGGCagcucaCCGgcgGCGGUGAu -3' miRNA: 3'- -CCUAGuCCaGCUGc-----GGCaa-CGCCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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