Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19050 | 3' | -63.9 | NC_004684.1 | + | 15512 | 0.67 | 0.30939 |
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Target: 5'- aAGGccGCCCGGGGC-CGGGcgcuGGA-GGCGc -3' miRNA: 3'- -UCCa-CGGGCCUCGcGUCC----CCUaCCGC- -5' |
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| 19050 | 3' | -63.9 | NC_004684.1 | + | 19775 | 0.67 | 0.30939 |
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Target: 5'- cGGUGCCCgccgGGGGCGaCAa--GAUGGCGg -3' miRNA: 3'- uCCACGGG----CCUCGC-GUcccCUACCGC- -5' |
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| 19050 | 3' | -63.9 | NC_004684.1 | + | 47414 | 0.66 | 0.323223 |
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Target: 5'- cGGUGCCUgcuGGAgGUGguGGGGGUugaucgcGGCc -3' miRNA: 3'- uCCACGGG---CCU-CGCguCCCCUA-------CCGc -5' |
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| 19050 | 3' | -63.9 | NC_004684.1 | + | 36985 | 0.66 | 0.323964 |
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Target: 5'- cAGGUguuGCCaCGGcAGCGCc-GGGAUGcGCGu -3' miRNA: 3'- -UCCA---CGG-GCC-UCGCGucCCCUAC-CGC- -5' |
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| 19050 | 3' | -63.9 | NC_004684.1 | + | 40450 | 0.66 | 0.339043 |
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Target: 5'- cGGUGCCCaGGcGGcCGCcugcucGGGGGUcucGGCGg -3' miRNA: 3'- uCCACGGG-CC-UC-GCGu-----CCCCUA---CCGC- -5' |
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| 19050 | 3' | -63.9 | NC_004684.1 | + | 56082 | 0.66 | 0.354625 |
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Target: 5'- gAGGUGCUgugCGGcaugcuGGCGguGGcGGUGGCa -3' miRNA: 3'- -UCCACGG---GCC------UCGCguCCcCUACCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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