Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19050 | 3' | -63.9 | NC_004684.1 | + | 56082 | 0.66 | 0.354625 |
Target: 5'- gAGGUGCUgugCGGcaugcuGGCGguGGcGGUGGCa -3' miRNA: 3'- -UCCACGG---GCC------UCGCguCCcCUACCGc -5' |
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19050 | 3' | -63.9 | NC_004684.1 | + | 56929 | 0.69 | 0.221276 |
Target: 5'- --cUGCCUGGAGCGCGcccGGGAcUGGUGc -3' miRNA: 3'- uccACGGGCCUCGCGUc--CCCU-ACCGC- -5' |
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19050 | 3' | -63.9 | NC_004684.1 | + | 57595 | 0.69 | 0.215878 |
Target: 5'- cGGGUGCCaccGGCGCAGGGccucGGCGu -3' miRNA: 3'- -UCCACGGgccUCGCGUCCCcua-CCGC- -5' |
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19050 | 3' | -63.9 | NC_004684.1 | + | 60734 | 0.68 | 0.244027 |
Target: 5'- gAGGUGCCCGGcgaccggguGGCcgccgGguGGGGGuUGGUc -3' miRNA: 3'- -UCCACGGGCC---------UCG-----CguCCCCU-ACCGc -5' |
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19050 | 3' | -63.9 | NC_004684.1 | + | 62153 | 0.69 | 0.231849 |
Target: 5'- -cGUGCCCGGccuuGCGCAGGGcucgcguGAcccggccgaugaUGGCGa -3' miRNA: 3'- ucCACGGGCCu---CGCGUCCC-------CU------------ACCGC- -5' |
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19050 | 3' | -63.9 | NC_004684.1 | + | 66637 | 0.68 | 0.268688 |
Target: 5'- cGGUGUCCaGGAGCGCcgccAGGGcGUcGGUGu -3' miRNA: 3'- uCCACGGG-CCUCGCG----UCCCcUA-CCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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