Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19209 | 5' | -57.5 | NC_004684.1 | + | 66845 | 0.67 | 0.615592 |
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Target: 5'- cCUCGgugGCGGCCuCGaCCGCCUGGc-- -3' miRNA: 3'- -GAGCacaUGCUGG-GC-GGUGGGCCaau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 61920 | 0.66 | 0.626258 |
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Target: 5'- aUCGcgggGUGCGggccacggcACCCGCCggGCCUGGUg- -3' miRNA: 3'- gAGCa---CAUGC---------UGGGCGG--UGGGCCAau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 66656 | 0.66 | 0.626258 |
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Target: 5'- cCUCGgcgGCGGCCCgGUCACCggCGGUg- -3' miRNA: 3'- -GAGCacaUGCUGGG-CGGUGG--GCCAau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 27621 | 0.66 | 0.63693 |
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Target: 5'- aUCGaGaACGGCCaggacgccaUGCCACCCGGUc- -3' miRNA: 3'- gAGCaCaUGCUGG---------GCGGUGGGCCAau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 42748 | 0.66 | 0.668882 |
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Target: 5'- -aCGUGguugGCGGCCUgGCCGgugcgaucCCCGGUUu -3' miRNA: 3'- gaGCACa---UGCUGGG-CGGU--------GGGCCAAu -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 5987 | 0.66 | 0.668882 |
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Target: 5'- gUCaUGUACggcgggacgGACCCGCCACCUGa--- -3' miRNA: 3'- gAGcACAUG---------CUGGGCGGUGGGCcaau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 42607 | 0.66 | 0.668882 |
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Target: 5'- cCUUGgccGUAuCGACCaGCgGCCCGGUg- -3' miRNA: 3'- -GAGCa--CAU-GCUGGgCGgUGGGCCAau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 66910 | 0.66 | 0.668882 |
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Target: 5'- gUCG-GUGCugccGACCuCGCUgGCCCGGUUGu -3' miRNA: 3'- gAGCaCAUG----CUGG-GCGG-UGGGCCAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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