Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19264 | 5' | -54.9 | NC_004685.1 | + | 3968 | 0.66 | 0.811594 |
Target: 5'- --gGCGCGguccagaaGUC---CGCGGGCAGa -3' miRNA: 3'- acaCGCGCaag-----CAGuuuGCGCCCGUC- -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 1906 | 0.66 | 0.811594 |
Target: 5'- cGUGCccgcugcggccGCGguuggCGUCAGcggcgacggGCGUGGGCAa -3' miRNA: 3'- aCACG-----------CGCaa---GCAGUU---------UGCGCCCGUc -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 59 | 0.66 | 0.782964 |
Target: 5'- --cGUGCGUUCGagCcGGgGCGGGCGc -3' miRNA: 3'- acaCGCGCAAGCa-GuUUgCGCCCGUc -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 40862 | 0.66 | 0.782964 |
Target: 5'- --aGCGCGgugcuggCGUCGcgggccGCGCGGGCc- -3' miRNA: 3'- acaCGCGCaa-----GCAGUu-----UGCGCCCGuc -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 64900 | 0.66 | 0.782964 |
Target: 5'- --gGCGCGUgc-UCGAAgUGCGGGCAc -3' miRNA: 3'- acaCGCGCAagcAGUUU-GCGCCCGUc -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 64861 | 0.66 | 0.773102 |
Target: 5'- cGUGCGCcgaagguagUCGuUCAGGCGaucaaGGGCAu -3' miRNA: 3'- aCACGCGca-------AGC-AGUUUGCg----CCCGUc -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 22272 | 0.67 | 0.763098 |
Target: 5'- gGUGCGC--UgGUCG---GCGGGCGGg -3' miRNA: 3'- aCACGCGcaAgCAGUuugCGCCCGUC- -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 49097 | 0.67 | 0.752963 |
Target: 5'- --cGCGCGUcgugcUCGUCG-ACGCaGuGCAGg -3' miRNA: 3'- acaCGCGCA-----AGCAGUuUGCGcC-CGUC- -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 18181 | 0.67 | 0.711335 |
Target: 5'- gGUGuCGUGUaCGUgGAGC-CGGGCAa -3' miRNA: 3'- aCAC-GCGCAaGCAgUUUGcGCCCGUc -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 62677 | 0.68 | 0.690022 |
Target: 5'- -uUGcCGCGacCGUC--GCGCGGGUAGc -3' miRNA: 3'- acAC-GCGCaaGCAGuuUGCGCCCGUC- -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 68352 | 0.68 | 0.690022 |
Target: 5'- gUGUGUGCGUgCGcC--GCGCGGGUc- -3' miRNA: 3'- -ACACGCGCAaGCaGuuUGCGCCCGuc -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 68432 | 0.68 | 0.679279 |
Target: 5'- --cGCGCGUagGUCcGAUGCGGGg-- -3' miRNA: 3'- acaCGCGCAagCAGuUUGCGCCCguc -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 919 | 0.68 | 0.679279 |
Target: 5'- gGUGauCGcCGUUCGUaCAGGCcgcaguccuGCGGGCGGa -3' miRNA: 3'- aCAC--GC-GCAAGCA-GUUUG---------CGCCCGUC- -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 18051 | 0.68 | 0.679279 |
Target: 5'- gGUG-GUG-UCGUUcGACGcCGGGCAGa -3' miRNA: 3'- aCACgCGCaAGCAGuUUGC-GCCCGUC- -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 32251 | 0.68 | 0.657673 |
Target: 5'- cUGaaCGCGgccgCGUCGgccgccgguGGCGCGGGCGGu -3' miRNA: 3'- -ACacGCGCaa--GCAGU---------UUGCGCCCGUC- -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 47537 | 0.68 | 0.657673 |
Target: 5'- gGUGaGCGcgCGgugccaGGugGCGGGCAGg -3' miRNA: 3'- aCACgCGCaaGCag----UUugCGCCCGUC- -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 37789 | 0.69 | 0.635974 |
Target: 5'- cUGUcgGUGUUCGUCAAaugggcugacuACGUGGGCAc -3' miRNA: 3'- -ACAcgCGCAAGCAGUU-----------UGCGCCCGUc -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 34002 | 0.69 | 0.625114 |
Target: 5'- aUGcUGCGCcg-CGUC-AACGUGGGCGa -3' miRNA: 3'- -AC-ACGCGcaaGCAGuUUGCGCCCGUc -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 34106 | 0.69 | 0.614261 |
Target: 5'- gGUGaa-GUUCGgcccccugGAGCGCGGGCAGg -3' miRNA: 3'- aCACgcgCAAGCag------UUUGCGCCCGUC- -5' |
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19264 | 5' | -54.9 | NC_004685.1 | + | 31688 | 0.69 | 0.614261 |
Target: 5'- gGUcGCGCccugCGUCAccaccaGGCGCGGGCGa -3' miRNA: 3'- aCA-CGCGcaa-GCAGU------UUGCGCCCGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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