Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19274 | 5' | -52.3 | NC_004685.1 | + | 24026 | 0.72 | 0.613995 |
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Target: 5'- gUCGGGCGUuCugGCGGUUgggcggCAGGuACa -3' miRNA: 3'- aGGUCCGCAuGugCGCCAAa-----GUCU-UG- -5' |
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| 19274 | 5' | -52.3 | NC_004685.1 | + | 58565 | 0.72 | 0.602936 |
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Target: 5'- aCCGGGCGUugaaccuaucGCGCcaagcacgaucGCGGUgUUCAGGGCu -3' miRNA: 3'- aGGUCCGCA----------UGUG-----------CGCCA-AAGUCUUG- -5' |
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| 19274 | 5' | -52.3 | NC_004685.1 | + | 38262 | 0.72 | 0.5897 |
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Target: 5'- aCCGGGCGgcaaaccguaggGCACGCGGUcaccgaaguUUcCAGGGCc -3' miRNA: 3'- aGGUCCGCa-----------UGUGCGCCA---------AA-GUCUUG- -5' |
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| 19274 | 5' | -52.3 | NC_004685.1 | + | 50100 | 0.74 | 0.495115 |
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Target: 5'- aCCAGGCGUguugaGCuuGCGGUcgUCAGAc- -3' miRNA: 3'- aGGUCCGCA-----UGugCGCCAa-AGUCUug -5' |
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| 19274 | 5' | -52.3 | NC_004685.1 | + | 10493 | 0.74 | 0.464404 |
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Target: 5'- gCCGGGUGUGCugGagugggCGGUcggUCAGGGCa -3' miRNA: 3'- aGGUCCGCAUGugC------GCCAa--AGUCUUG- -5' |
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| 19274 | 5' | -52.3 | NC_004685.1 | + | 178 | 0.78 | 0.289528 |
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Target: 5'- cCCuugcgcGGCGUGCGCGCGgGUUUCGGcGCg -3' miRNA: 3'- aGGu-----CCGCAUGUGCGC-CAAAGUCuUG- -5' |
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| 19274 | 5' | -52.3 | NC_004685.1 | + | 66351 | 1.1 | 0.0023 |
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Target: 5'- aUCCAGGCGUACACGCGGUUUCAGAACc -3' miRNA: 3'- -AGGUCCGCAUGUGCGCCAAAGUCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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