Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19564 | 3' | -59.7 | NC_004686.1 | + | 3803 | 0.66 | 0.509481 |
Target: 5'- cCGCcauGGCGGCUUCgCUCc-ACGUCuCCAa -3' miRNA: 3'- -GCG---CCGCCGAAG-GAGcuUGCGGuGGU- -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 5271 | 0.66 | 0.503427 |
Target: 5'- uGCGGCucugucggguucgccGGCgaagaUCCUCGA-CaCCACCAa -3' miRNA: 3'- gCGCCG---------------CCGa----AGGAGCUuGcGGUGGU- -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 38639 | 0.66 | 0.489424 |
Target: 5'- --aGGUGGCgUUCUUCGu-CGCCGCUg -3' miRNA: 3'- gcgCCGCCG-AAGGAGCuuGCGGUGGu -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 18831 | 0.66 | 0.469743 |
Target: 5'- gGCGGCGGUgcgCgaCGAGa-CCGCCAa -3' miRNA: 3'- gCGCCGCCGaa-GgaGCUUgcGGUGGU- -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 21847 | 0.66 | 0.469743 |
Target: 5'- gCGCgGGCGGCggCUcCGGACGgCGCUc -3' miRNA: 3'- -GCG-CCGCCGaaGGaGCUUGCgGUGGu -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 7009 | 0.66 | 0.469743 |
Target: 5'- uGUGGUGGCg--UUCGAGgaaGCCGCCGc -3' miRNA: 3'- gCGCCGCCGaagGAGCUUg--CGGUGGU- -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 47307 | 0.66 | 0.468769 |
Target: 5'- uGCGccgccuacgacccGUGGUUUCCgcagcCGAaccGCGCCACCGu -3' miRNA: 3'- gCGC-------------CGCCGAAGGa----GCU---UGCGGUGGU- -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 22138 | 0.66 | 0.466825 |
Target: 5'- uCGCGGUGaGCgacacgCCacccgaggugaaugUCGAcGCGCCGCCGu -3' miRNA: 3'- -GCGCCGC-CGaa----GG--------------AGCU-UGCGGUGGU- -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 51528 | 0.67 | 0.460055 |
Target: 5'- uCGCGGCGugggacGCg--CUC-AACGCCGCCAc -3' miRNA: 3'- -GCGCCGC------CGaagGAGcUUGCGGUGGU- -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 51731 | 0.67 | 0.460055 |
Target: 5'- uGCGGCGGCaccgaCCUCuucagGGACGUCGgCGa -3' miRNA: 3'- gCGCCGCCGaa---GGAG-----CUUGCGGUgGU- -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 39839 | 0.67 | 0.460055 |
Target: 5'- cCGCGGCguccguguaggaGGCgccgUCgUCGAGCaggcggGCCGCCu -3' miRNA: 3'- -GCGCCG------------CCGa---AGgAGCUUG------CGGUGGu -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 21752 | 0.68 | 0.39542 |
Target: 5'- gGUGuGCGuGCUUCCccaccaGGACGCCcACCAu -3' miRNA: 3'- gCGC-CGC-CGAAGGag----CUUGCGG-UGGU- -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 37051 | 0.68 | 0.386674 |
Target: 5'- cCGUGGuUGGCgcgCCUgGAGCGUCagGCCAu -3' miRNA: 3'- -GCGCC-GCCGaa-GGAgCUUGCGG--UGGU- -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 611 | 0.68 | 0.361215 |
Target: 5'- aGCuGCGGCUucUCCUCaAACGCC-UCAg -3' miRNA: 3'- gCGcCGCCGA--AGGAGcUUGCGGuGGU- -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 44806 | 0.68 | 0.361215 |
Target: 5'- uGCGGCGGUgauguacacggUUUCUgGGACGUgGCCc -3' miRNA: 3'- gCGCCGCCG-----------AAGGAgCUUGCGgUGGu -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 51166 | 0.69 | 0.344902 |
Target: 5'- uCGCGGCGGacga---GAAUGCCGCCGc -3' miRNA: 3'- -GCGCCGCCgaaggagCUUGCGGUGGU- -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 40196 | 0.69 | 0.336946 |
Target: 5'- aGUGGuCGGCgUUCacgCUCGGACagGCCGCCGa -3' miRNA: 3'- gCGCC-GCCG-AAG---GAGCUUG--CGGUGGU- -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 22124 | 0.69 | 0.336946 |
Target: 5'- aGUGGCGcGCgcggCCUCG-GCGgCACCGc -3' miRNA: 3'- gCGCCGC-CGaa--GGAGCuUGCgGUGGU- -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 12271 | 0.69 | 0.329124 |
Target: 5'- aGCGGCGuuaGCacccaacCCgauugCGAACGCCGCCGc -3' miRNA: 3'- gCGCCGC---CGaa-----GGa----GCUUGCGGUGGU- -5' |
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19564 | 3' | -59.7 | NC_004686.1 | + | 41411 | 0.69 | 0.320677 |
Target: 5'- aGCGGCgaggucucgGGCUUCCUccacCGAaucgagaugggcaGCGCCGCUg -3' miRNA: 3'- gCGCCG---------CCGAAGGA----GCU-------------UGCGGUGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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