Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19744 | 3' | -60.5 | NC_004687.1 | + | 38535 | 0.71 | 0.415588 |
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Target: 5'- gAGGUCGGCCAUGUCGacgaccgaGC-GcGGCCUg -3' miRNA: 3'- -UCCAGCCGGUGCAGUa-------CGaC-CCGGAg -5' |
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| 19744 | 3' | -60.5 | NC_004687.1 | + | 85105 | 0.72 | 0.382335 |
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Target: 5'- --uUCGGCCACGc---GCUGGGCCUg -3' miRNA: 3'- uccAGCCGGUGCaguaCGACCCGGAg -5' |
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| 19744 | 3' | -60.5 | NC_004687.1 | + | 5480 | 0.72 | 0.350901 |
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Target: 5'- gAGGaCGGCgACGUCuu-CUGGGCCUa -3' miRNA: 3'- -UCCaGCCGgUGCAGuacGACCCGGAg -5' |
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| 19744 | 3' | -60.5 | NC_004687.1 | + | 29845 | 0.72 | 0.343335 |
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Target: 5'- cGGGUCagcgGGaCCACGagCAUGUUGGGCCg- -3' miRNA: 3'- -UCCAG----CC-GGUGCa-GUACGACCCGGag -5' |
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| 19744 | 3' | -60.5 | NC_004687.1 | + | 102187 | 0.73 | 0.307283 |
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Target: 5'- cGGGacgaUCGGCCACGcUCAgccGgaGGGCCUUg -3' miRNA: 3'- -UCC----AGCCGGUGC-AGUa--CgaCCCGGAG- -5' |
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| 19744 | 3' | -60.5 | NC_004687.1 | + | 150777 | 0.76 | 0.221924 |
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Target: 5'- uGGUUGGCCAUGUCcuggGCguaGGCCUCa -3' miRNA: 3'- uCCAGCCGGUGCAGua--CGac-CCGGAG- -5' |
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| 19744 | 3' | -60.5 | NC_004687.1 | + | 110951 | 0.79 | 0.128892 |
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Target: 5'- gGGGUCGGCgAUGUUGuucUGCUGGGCCa- -3' miRNA: 3'- -UCCAGCCGgUGCAGU---ACGACCCGGag -5' |
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| 19744 | 3' | -60.5 | NC_004687.1 | + | 142653 | 1.09 | 0.00098 |
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Target: 5'- cAGGUCGGCCACGUCAUGCUGGGCCUCg -3' miRNA: 3'- -UCCAGCCGGUGCAGUACGACCCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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