Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20405 | 5' | -55.3 | NC_004688.1 | + | 26059 | 0.66 | 0.906553 |
Target: 5'- -cGCCCGcagcaccggGGGcacGGCUCCGGGGagGCCc -3' miRNA: 3'- gcUGGGUa--------UUC---UCGAGGCCUCagCGG- -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 73653 | 0.66 | 0.906553 |
Target: 5'- aGGCCCucgucgcGGAGCcucuuguacUCCucGAGUCGCCc -3' miRNA: 3'- gCUGGGuau----UCUCG---------AGGc-CUCAGCGG- -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 385 | 0.66 | 0.906553 |
Target: 5'- -cACCCAUuGGGGCgCUGaaagaAGUCGCCa -3' miRNA: 3'- gcUGGGUAuUCUCGaGGCc----UCAGCGG- -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 48887 | 0.66 | 0.886683 |
Target: 5'- uGugCCGc-GGGGCUCCGGuGgaGCCc -3' miRNA: 3'- gCugGGUauUCUCGAGGCCuCagCGG- -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 30426 | 0.66 | 0.878868 |
Target: 5'- uCGACCCAgucgguguAGAGUugaccguUgCGGAuaaugGUCGCCa -3' miRNA: 3'- -GCUGGGUau------UCUCG-------AgGCCU-----CAGCGG- -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 67177 | 0.67 | 0.864725 |
Target: 5'- uCGACCCAguGGA-CUucgUCGGAGUCaagGCCg -3' miRNA: 3'- -GCUGGGUauUCUcGA---GGCCUCAG---CGG- -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 91862 | 0.67 | 0.864725 |
Target: 5'- gGACCCAUucGAGGG-UCCGcGA-UUGCCa -3' miRNA: 3'- gCUGGGUA--UUCUCgAGGC-CUcAGCGG- -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 85676 | 0.67 | 0.848996 |
Target: 5'- gCGACCCAUGu-AGUcaUGGAGuUCGCCc -3' miRNA: 3'- -GCUGGGUAUucUCGagGCCUC-AGCGG- -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 80082 | 0.67 | 0.848996 |
Target: 5'- uGG-CCAUAGGGGCaUUCGGuG-CGCCg -3' miRNA: 3'- gCUgGGUAUUCUCG-AGGCCuCaGCGG- -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 11865 | 0.67 | 0.840823 |
Target: 5'- uCGACCCAccccguGGAGUagcgacagaucUUCGGGGgccCGCCa -3' miRNA: 3'- -GCUGGGUau----UCUCG-----------AGGCCUCa--GCGG- -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 100061 | 0.67 | 0.829054 |
Target: 5'- uGGCCUAcgcgcucgcaacaGAGAGCgUCCGGguGGUUGCCc -3' miRNA: 3'- gCUGGGUa------------UUCUCG-AGGCC--UCAGCGG- -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 49400 | 0.67 | 0.823031 |
Target: 5'- gCGGCCCAcGAGAauggggcGUUCCau-GUCGCCg -3' miRNA: 3'- -GCUGGGUaUUCU-------CGAGGccuCAGCGG- -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 84815 | 0.67 | 0.823031 |
Target: 5'- gCGACCCAU-----CUCCGGGGUCcacuucaGCCc -3' miRNA: 3'- -GCUGGGUAuucucGAGGCCUCAG-------CGG- -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 61809 | 0.68 | 0.81516 |
Target: 5'- gGGCUCGUcGGAGCUgCGG-GUCcagcgGCCg -3' miRNA: 3'- gCUGGGUAuUCUCGAgGCCuCAG-----CGG- -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 36620 | 0.68 | 0.81516 |
Target: 5'- uGACaCCAguaccGAcGCUCCGGcGGUgGCCg -3' miRNA: 3'- gCUG-GGUauu--CU-CGAGGCC-UCAgCGG- -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 95516 | 0.68 | 0.797179 |
Target: 5'- gCGGCCa---GGGGCcgCCGGAGUgGCg -3' miRNA: 3'- -GCUGGguauUCUCGa-GGCCUCAgCGg -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 53643 | 0.69 | 0.759443 |
Target: 5'- -cGCCCA----GGCUCCGGcauugacGUCGCCg -3' miRNA: 3'- gcUGGGUauucUCGAGGCCu------CAGCGG- -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 72279 | 0.69 | 0.759443 |
Target: 5'- gCGAuCCCGgcuucGGuaUCCGGGGUUGCCu -3' miRNA: 3'- -GCU-GGGUauu--CUcgAGGCCUCAGCGG- -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 80327 | 0.69 | 0.759443 |
Target: 5'- -aACUCGcUAAGAcGCcCCGGcAGUCGCCg -3' miRNA: 3'- gcUGGGU-AUUCU-CGaGGCC-UCAGCGG- -5' |
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20405 | 5' | -55.3 | NC_004688.1 | + | 65717 | 0.69 | 0.729886 |
Target: 5'- gGAacUCCuc-GGGGCaacgCCGGGGUCGCCc -3' miRNA: 3'- gCU--GGGuauUCUCGa---GGCCUCAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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