Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20405 | 5' | -55.3 | NC_004688.1 | + | 93218 | 0.7 | 0.709728 |
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Target: 5'- cCGACCuCGgcgAAGGGCUCgGcGGGcUUGCCa -3' miRNA: 3'- -GCUGG-GUa--UUCUCGAGgC-CUC-AGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 519 | 0.7 | 0.668672 |
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Target: 5'- uGcCCCAgguGAGCUCgccacaGGAGUCGCa -3' miRNA: 3'- gCuGGGUauuCUCGAGg-----CCUCAGCGg -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 68778 | 0.71 | 0.620881 |
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Target: 5'- aCGGCCCcgAAGGGCUaCCGGAuauaucaacaacgagGUguccCGCCu -3' miRNA: 3'- -GCUGGGuaUUCUCGA-GGCCU---------------CA----GCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 81602 | 0.72 | 0.595969 |
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Target: 5'- uGACuCCAUGGGGGCUCCuaug-CGCCg -3' miRNA: 3'- gCUG-GGUAUUCUCGAGGccucaGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 96884 | 0.72 | 0.575325 |
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Target: 5'- aGACCCG--AGAaaacccaaUUCCGGAGUUGCCg -3' miRNA: 3'- gCUGGGUauUCUc-------GAGGCCUCAGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 42006 | 0.72 | 0.565062 |
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Target: 5'- cCGGCCU----GGGCggCGGGGUCGCCg -3' miRNA: 3'- -GCUGGGuauuCUCGagGCCUCAGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 25604 | 0.74 | 0.44732 |
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Target: 5'- uGGCCUgc-GGAGaUCCGGGGUUGCCg -3' miRNA: 3'- gCUGGGuauUCUCgAGGCCUCAGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 5832 | 0.76 | 0.368549 |
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Target: 5'- uGACCCAccacguAGAGC-CUGGAGUCGUUg -3' miRNA: 3'- gCUGGGUau----UCUCGaGGCCUCAGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 1218 | 1.11 | 0.001958 |
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Target: 5'- uCGACCCAUAAGAGCUCCGGAGUCGCCc -3' miRNA: 3'- -GCUGGGUAUUCUCGAGGCCUCAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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