Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 20405 | 5' | -55.3 | NC_004688.1 | + | 80327 | 0.69 | 0.759443 |
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Target: 5'- -aACUCGcUAAGAcGCcCCGGcAGUCGCCg -3' miRNA: 3'- gcUGGGU-AUUCU-CGaGGCC-UCAGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 81602 | 0.72 | 0.595969 |
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Target: 5'- uGACuCCAUGGGGGCUCCuaug-CGCCg -3' miRNA: 3'- gCUG-GGUAUUCUCGAGGccucaGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 84815 | 0.67 | 0.823031 |
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Target: 5'- gCGACCCAU-----CUCCGGGGUCcacuucaGCCc -3' miRNA: 3'- -GCUGGGUAuucucGAGGCCUCAG-------CGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 85676 | 0.67 | 0.848996 |
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Target: 5'- gCGACCCAUGu-AGUcaUGGAGuUCGCCc -3' miRNA: 3'- -GCUGGGUAUucUCGagGCCUC-AGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 91862 | 0.67 | 0.864725 |
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Target: 5'- gGACCCAUucGAGGG-UCCGcGA-UUGCCa -3' miRNA: 3'- gCUGGGUA--UUCUCgAGGC-CUcAGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 93218 | 0.7 | 0.709728 |
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Target: 5'- cCGACCuCGgcgAAGGGCUCgGcGGGcUUGCCa -3' miRNA: 3'- -GCUGG-GUa--UUCUCGAGgC-CUC-AGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 95516 | 0.68 | 0.797179 |
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Target: 5'- gCGGCCa---GGGGCcgCCGGAGUgGCg -3' miRNA: 3'- -GCUGGguauUCUCGa-GGCCUCAgCGg -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 96884 | 0.72 | 0.575325 |
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Target: 5'- aGACCCG--AGAaaacccaaUUCCGGAGUUGCCg -3' miRNA: 3'- gCUGGGUauUCUc-------GAGGCCUCAGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 100061 | 0.67 | 0.829054 |
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Target: 5'- uGGCCUAcgcgcucgcaacaGAGAGCgUCCGGguGGUUGCCc -3' miRNA: 3'- gCUGGGUa------------UUCUCG-AGGCC--UCAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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