Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 20405 | 5' | -55.3 | NC_004688.1 | + | 81602 | 0.72 | 0.595969 |
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Target: 5'- uGACuCCAUGGGGGCUCCuaug-CGCCg -3' miRNA: 3'- gCUG-GGUAUUCUCGAGGccucaGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 68778 | 0.71 | 0.620881 |
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Target: 5'- aCGGCCCcgAAGGGCUaCCGGAuauaucaacaacgagGUguccCGCCu -3' miRNA: 3'- -GCUGGGuaUUCUCGA-GGCCU---------------CA----GCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 519 | 0.7 | 0.668672 |
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Target: 5'- uGcCCCAgguGAGCUCgccacaGGAGUCGCa -3' miRNA: 3'- gCuGGGUauuCUCGAGg-----CCUCAGCGg -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 93218 | 0.7 | 0.709728 |
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Target: 5'- cCGACCuCGgcgAAGGGCUCgGcGGGcUUGCCa -3' miRNA: 3'- -GCUGG-GUa--UUCUCGAGgC-CUC-AGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 65717 | 0.69 | 0.729886 |
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Target: 5'- gGAacUCCuc-GGGGCaacgCCGGGGUCGCCc -3' miRNA: 3'- gCU--GGGuauUCUCGa---GGCCUCAGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 80327 | 0.69 | 0.759443 |
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Target: 5'- -aACUCGcUAAGAcGCcCCGGcAGUCGCCg -3' miRNA: 3'- gcUGGGU-AUUCU-CGaGGCC-UCAGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 72279 | 0.69 | 0.759443 |
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Target: 5'- gCGAuCCCGgcuucGGuaUCCGGGGUUGCCu -3' miRNA: 3'- -GCU-GGGUauu--CUcgAGGCCUCAGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 53643 | 0.69 | 0.759443 |
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Target: 5'- -cGCCCA----GGCUCCGGcauugacGUCGCCg -3' miRNA: 3'- gcUGGGUauucUCGAGGCCu------CAGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 26059 | 0.66 | 0.906553 |
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Target: 5'- -cGCCCGcagcaccggGGGcacGGCUCCGGGGagGCCc -3' miRNA: 3'- gcUGGGUa--------UUC---UCGAGGCCUCagCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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