Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20405 | 5' | -55.3 | NC_004688.1 | + | 80082 | 0.67 | 0.848996 |
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Target: 5'- uGG-CCAUAGGGGCaUUCGGuG-CGCCg -3' miRNA: 3'- gCUgGGUAUUCUCG-AGGCCuCaGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 85676 | 0.67 | 0.848996 |
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Target: 5'- gCGACCCAUGu-AGUcaUGGAGuUCGCCc -3' miRNA: 3'- -GCUGGGUAUucUCGagGCCUC-AGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 67177 | 0.67 | 0.864725 |
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Target: 5'- uCGACCCAguGGA-CUucgUCGGAGUCaagGCCg -3' miRNA: 3'- -GCUGGGUauUCUcGA---GGCCUCAG---CGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 91862 | 0.67 | 0.864725 |
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Target: 5'- gGACCCAUucGAGGG-UCCGcGA-UUGCCa -3' miRNA: 3'- gCUGGGUA--UUCUCgAGGC-CUcAGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 30426 | 0.66 | 0.878868 |
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Target: 5'- uCGACCCAgucgguguAGAGUugaccguUgCGGAuaaugGUCGCCa -3' miRNA: 3'- -GCUGGGUau------UCUCG-------AgGCCU-----CAGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 48887 | 0.66 | 0.886683 |
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Target: 5'- uGugCCGc-GGGGCUCCGGuGgaGCCc -3' miRNA: 3'- gCugGGUauUCUCGAGGCCuCagCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 385 | 0.66 | 0.906553 |
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Target: 5'- -cACCCAUuGGGGCgCUGaaagaAGUCGCCa -3' miRNA: 3'- gcUGGGUAuUCUCGaGGCc----UCAGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 73653 | 0.66 | 0.906553 |
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Target: 5'- aGGCCCucgucgcGGAGCcucuuguacUCCucGAGUCGCCc -3' miRNA: 3'- gCUGGGuau----UCUCG---------AGGc-CUCAGCGG- -5' |
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| 20405 | 5' | -55.3 | NC_004688.1 | + | 26059 | 0.66 | 0.906553 |
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Target: 5'- -cGCCCGcagcaccggGGGcacGGCUCCGGGGagGCCc -3' miRNA: 3'- gcUGGGUa--------UUC---UCGAGGCCUCagCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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