Results 1 - 20 of 42 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20415 | 5' | -59.9 | NC_004688.1 | + | 37084 | 0.66 | 0.692905 |
Target: 5'- cGCCauGGCGGcCCAUgugCCCGAcgagcuuGUCGCc -3' miRNA: 3'- -CGGcuCCGCCaGGUG---GGGCUu------CAGCG- -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 31635 | 0.66 | 0.692905 |
Target: 5'- gGCCGAGGUcuuGGgcgCCucCCCCGugguGGggggCGCc -3' miRNA: 3'- -CGGCUCCG---CCa--GGu-GGGGCu---UCa---GCG- -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 43881 | 0.66 | 0.692905 |
Target: 5'- aGCuCGcGcGCGaGaUCCGCCCCGAAGcCGg -3' miRNA: 3'- -CG-GCuC-CGC-C-AGGUGGGGCUUCaGCg -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 9582 | 0.66 | 0.682993 |
Target: 5'- cGCCGcgguguAGGCcucGGUugCCGCCCgGAGGUCu- -3' miRNA: 3'- -CGGC------UCCG---CCA--GGUGGGgCUUCAGcg -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 39986 | 0.66 | 0.67304 |
Target: 5'- uGuuGuGGC-GUCCGCCgCCGcAGUgGCg -3' miRNA: 3'- -CggCuCCGcCAGGUGG-GGCuUCAgCG- -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 4890 | 0.66 | 0.67304 |
Target: 5'- cGCCcauGGUGGUCCACCCgCGc--UCGg -3' miRNA: 3'- -CGGcu-CCGCCAGGUGGG-GCuucAGCg -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 9748 | 0.66 | 0.66905 |
Target: 5'- uGCCGAGGagugcuucgacuuCGGcgacggucaguucgUCgGCCUCGggGUCGa -3' miRNA: 3'- -CGGCUCC-------------GCC--------------AGgUGGGGCuuCAGCg -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 43137 | 0.67 | 0.663055 |
Target: 5'- uGUCGAGGCGGUuauuggugggaUCGCCaUCGuuGUgGCa -3' miRNA: 3'- -CGGCUCCGCCA-----------GGUGG-GGCuuCAgCG- -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 7726 | 0.67 | 0.653046 |
Target: 5'- cGUCGAuGCGGUagaCCGgCUCGAuccAGUCGCc -3' miRNA: 3'- -CGGCUcCGCCA---GGUgGGGCU---UCAGCG- -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 91493 | 0.67 | 0.643021 |
Target: 5'- uCCGAGGCGGgu-GCCgCGGAuGUCGa -3' miRNA: 3'- cGGCUCCGCCaggUGGgGCUU-CAGCg -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 20311 | 0.67 | 0.643021 |
Target: 5'- uGCCGAGGC---CgACCgCGggGUCGg -3' miRNA: 3'- -CGGCUCCGccaGgUGGgGCuuCAGCg -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 73355 | 0.67 | 0.643021 |
Target: 5'- uGCCGAGGCGcucggCCaaguggacgACCgUGAAGcCGCg -3' miRNA: 3'- -CGGCUCCGCca---GG---------UGGgGCUUCaGCG- -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 8831 | 0.67 | 0.643021 |
Target: 5'- uGUCGAGGCGGgCCACgaccuucaggCCCuu-GUCGUc -3' miRNA: 3'- -CGGCUCCGCCaGGUG----------GGGcuuCAGCG- -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 4749 | 0.67 | 0.622954 |
Target: 5'- uGCCGuucuGGCGGgacaCCACCaggCCGAAGgaggaggcCGCc -3' miRNA: 3'- -CGGCu---CCGCCa---GGUGG---GGCUUCa-------GCG- -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 30777 | 0.67 | 0.619945 |
Target: 5'- aGCCGcccucGGGCgucagaacaagauaGG-CCACCCCGguGUCGg -3' miRNA: 3'- -CGGC-----UCCG--------------CCaGGUGGGGCuuCAGCg -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 95522 | 0.67 | 0.612927 |
Target: 5'- uCCuAGGCGG-CCagggGCCgCCGGAGUgGCg -3' miRNA: 3'- cGGcUCCGCCaGG----UGG-GGCUUCAgCG- -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 22430 | 0.67 | 0.60892 |
Target: 5'- cGgCGAGGCGGauuUCCuCCCUGGucugcuuaugggcGUCGCu -3' miRNA: 3'- -CgGCUCCGCC---AGGuGGGGCUu------------CAGCG- -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 94960 | 0.68 | 0.601914 |
Target: 5'- gGCCGGGGCauaaagaaugugGGUaCCcucgugGCCCUcuucaaggucgcaGAAGUCGCa -3' miRNA: 3'- -CGGCUCCG------------CCA-GG------UGGGG-------------CUUCAGCG- -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 43538 | 0.68 | 0.601914 |
Target: 5'- cGCCGAGGgGGU-CGCCgCGAcgGGcuucccguuaaucUCGCg -3' miRNA: 3'- -CGGCUCCgCCAgGUGGgGCU--UC-------------AGCG- -5' |
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20415 | 5' | -59.9 | NC_004688.1 | + | 28422 | 0.68 | 0.582957 |
Target: 5'- uGCCGGGGCcGUacaCCACCagguccgggaagCCGGgcuGGUCGCc -3' miRNA: 3'- -CGGCUCCGcCA---GGUGG------------GGCU---UCAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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