Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2066 | 3' | -48.4 | NC_001348.1 | + | 105258 | 0.68 | 0.9901 |
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Target: 5'- gGGCGcg-GGGGGAGGagcccCCCGCa -3' miRNA: 3'- gCCGUacaUUCCCUUCauuuuGGGCG- -5' |
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| 2066 | 3' | -48.4 | NC_001348.1 | + | 105872 | 0.69 | 0.985338 |
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Target: 5'- gGGCGacaGGGGGAcgcgGGUcuuGGGGCCCGCg -3' miRNA: 3'- gCCGUacaUUCCCU----UCA---UUUUGGGCG- -5' |
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| 2066 | 3' | -48.4 | NC_001348.1 | + | 108034 | 0.71 | 0.96059 |
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Target: 5'- cCGGCGcGUuucgguGGGAAGUGAAACUacagGCa -3' miRNA: 3'- -GCCGUaCAuu----CCCUUCAUUUUGGg---CG- -5' |
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| 2066 | 3' | -48.4 | NC_001348.1 | + | 108520 | 0.67 | 0.997121 |
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Target: 5'- gCGGgGgauacAGGGGAG-AAGGCCUGCg -3' miRNA: 3'- -GCCgUacau-UCCCUUCaUUUUGGGCG- -5' |
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| 2066 | 3' | -48.4 | NC_001348.1 | + | 115172 | 0.67 | 0.996225 |
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Target: 5'- cCGGCAcgaagaucaagcauaUGUugaaacAGGGAGGUAccGGACCCu- -3' miRNA: 3'- -GCCGU---------------ACAu-----UCCCUUCAU--UUUGGGcg -5' |
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| 2066 | 3' | -48.4 | NC_001348.1 | + | 121272 | 0.67 | 0.997121 |
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Target: 5'- gCGGgGgauacAGGGGAG-AAGGCCUGCg -3' miRNA: 3'- -GCCgUacau-UCCCUUCaUUUUGGGCG- -5' |
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| 2066 | 3' | -48.4 | NC_001348.1 | + | 121757 | 0.71 | 0.96059 |
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Target: 5'- cCGGCGcGUuucgguGGGAAGUGAAACUacagGCa -3' miRNA: 3'- -GCCGUaCAuu----CCCUUCAUUUUGGg---CG- -5' |
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| 2066 | 3' | -48.4 | NC_001348.1 | + | 123919 | 0.67 | 0.995969 |
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Target: 5'- gGGCGacaGGGGGAcgcgGGUcuuGGGGCCCGCc -3' miRNA: 3'- gCCGUacaUUCCCU----UCA---UUUUGGGCG- -5' |
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| 2066 | 3' | -48.4 | NC_001348.1 | + | 124534 | 0.68 | 0.9901 |
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Target: 5'- gGGCGcg-GGGGGAGGagcccCCCGCa -3' miRNA: 3'- gCCGUacaUUCCCUUCauuuuGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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