Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2066 | 5' | -58.7 | NC_001348.1 | + | 109811 | 0.68 | 0.615109 |
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Target: 5'- cGGcGGGGUaCCGc-GCCCCCUCC-CCa -3' miRNA: 3'- cCC-CCUCA-GGUuaUGGGGGAGGuGGa -5' |
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| 2066 | 5' | -58.7 | NC_001348.1 | + | 51759 | 0.68 | 0.625245 |
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Target: 5'- gGGGGGAGUac--UGCCCCCUCgugauuuuCAUCa -3' miRNA: 3'- -CCCCCUCAgguuAUGGGGGAG--------GUGGa -5' |
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| 2066 | 5' | -58.7 | NC_001348.1 | + | 32500 | 0.68 | 0.655653 |
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Target: 5'- cGGGGGAGU--AAUACCCCaucuugaaUCCAUa- -3' miRNA: 3'- -CCCCCUCAggUUAUGGGGg-------AGGUGga -5' |
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| 2066 | 5' | -58.7 | NC_001348.1 | + | 122561 | 0.66 | 0.761139 |
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Target: 5'- uGGGGGAGagcCCAGaggGCCUCCcccguggccauggcUUCGCCUa -3' miRNA: 3'- -CCCCCUCa--GGUUa--UGGGGG--------------AGGUGGA- -5' |
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| 2066 | 5' | -58.7 | NC_001348.1 | + | 118675 | 0.66 | 0.763962 |
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Target: 5'- cGGGGauGGGUgCAAcACCCCgUCC-CCa -3' miRNA: 3'- -CCCC--CUCAgGUUaUGGGGgAGGuGGa -5' |
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| 2066 | 5' | -58.7 | NC_001348.1 | + | 103224 | 1.09 | 0.001387 |
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Target: 5'- aGGGGGAGUCCAAUACCCCCUCCACCUc -3' miRNA: 3'- -CCCCCUCAGGUUAUGGGGGAGGUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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