Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 20753 | 5' | -51.8 | NC_004688.1 | + | 55816 | 0.67 | 0.930218 |
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Target: 5'- gGUGGGCGCCgcGAC-CgGCGGC--UAGCg -3' miRNA: 3'- -UAUCUGUGG--CUGaGgCGUCGuaAUCG- -5' |
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| 20753 | 5' | -51.8 | NC_004688.1 | + | 59263 | 0.68 | 0.918689 |
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Target: 5'- --cGACACCG-UUgUGCGGCAUgAGCc -3' miRNA: 3'- uauCUGUGGCuGAgGCGUCGUAaUCG- -5' |
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| 20753 | 5' | -51.8 | NC_004688.1 | + | 61801 | 0.66 | 0.958384 |
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Target: 5'- -cGGAgCugCGGgUCCaGCGGCcgUAGUg -3' miRNA: 3'- uaUCU-GugGCUgAGG-CGUCGuaAUCG- -5' |
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| 20753 | 5' | -51.8 | NC_004688.1 | + | 62957 | 0.68 | 0.906084 |
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Target: 5'- -gAGAuCACCGcguGCUCCGCGGUugaGGUa -3' miRNA: 3'- uaUCU-GUGGC---UGAGGCGUCGuaaUCG- -5' |
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| 20753 | 5' | -51.8 | NC_004688.1 | + | 65382 | 0.74 | 0.611598 |
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Target: 5'- --cGAUGCCGGCUUCGguGCcgUGGUc -3' miRNA: 3'- uauCUGUGGCUGAGGCguCGuaAUCG- -5' |
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| 20753 | 5' | -51.8 | NC_004688.1 | + | 81824 | 0.66 | 0.962172 |
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Target: 5'- uUGGAUugCGGCaaggUCCGCcauGGCGU-AGCc -3' miRNA: 3'- uAUCUGugGCUG----AGGCG---UCGUAaUCG- -5' |
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| 20753 | 5' | -51.8 | NC_004688.1 | + | 90298 | 0.66 | 0.965713 |
|
Target: 5'- -cGGGCACCGGgUCCG-GGUAUuucucgacaaUGGCu -3' miRNA: 3'- uaUCUGUGGCUgAGGCgUCGUA----------AUCG- -5' |
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| 20753 | 5' | -51.8 | NC_004688.1 | + | 93226 | 0.69 | 0.853842 |
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Target: 5'- cGUGGGCACCGAcCUCgGCgaagGGCuc-GGCg -3' miRNA: 3'- -UAUCUGUGGCU-GAGgCG----UCGuaaUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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