Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20775 | 5' | -54.5 | NC_004689.1 | + | 23562 | 0.66 | 0.796106 |
Target: 5'- uUCGCUCGGCCUGccgGAGUGucauucacacUCUGC-Cg -3' miRNA: 3'- -AGCGAGUCGGGUua-CUCGU----------AGACGcG- -5' |
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20775 | 5' | -54.5 | NC_004689.1 | + | 20764 | 0.66 | 0.795137 |
Target: 5'- cUCGCUCGaaacGCCCAGacggucgaacgcUGccuuugcuuucucAGCGUCUuGCGCc -3' miRNA: 3'- -AGCGAGU----CGGGUU------------AC-------------UCGUAGA-CGCG- -5' |
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20775 | 5' | -54.5 | NC_004689.1 | + | 58918 | 0.66 | 0.786337 |
Target: 5'- cUGCUCAGCgaCCGAUG-GCAgaUGCGa -3' miRNA: 3'- aGCGAGUCG--GGUUACuCGUagACGCg -5' |
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20775 | 5' | -54.5 | NC_004689.1 | + | 12299 | 0.66 | 0.776407 |
Target: 5'- aUCGcCUCGaCCUGAcGAGCGUCgGCGUc -3' miRNA: 3'- -AGC-GAGUcGGGUUaCUCGUAGaCGCG- -5' |
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20775 | 5' | -54.5 | NC_004689.1 | + | 36180 | 0.67 | 0.756113 |
Target: 5'- aCGCgCAGCCCu---AGCc-CUGCGCa -3' miRNA: 3'- aGCGaGUCGGGuuacUCGuaGACGCG- -5' |
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20775 | 5' | -54.5 | NC_004689.1 | + | 51127 | 0.67 | 0.735314 |
Target: 5'- gUCGaa-AGCCaccucguugUAAUGcGCGUCUGCGCa -3' miRNA: 3'- -AGCgagUCGG---------GUUACuCGUAGACGCG- -5' |
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20775 | 5' | -54.5 | NC_004689.1 | + | 46332 | 0.67 | 0.724754 |
Target: 5'- gCGUUCGG-CgAAUGAGgAUCggGCGCa -3' miRNA: 3'- aGCGAGUCgGgUUACUCgUAGa-CGCG- -5' |
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20775 | 5' | -54.5 | NC_004689.1 | + | 38933 | 0.68 | 0.703368 |
Target: 5'- aUGC-CAGCCgCucUGAuacGCGUCUGCGUu -3' miRNA: 3'- aGCGaGUCGG-GuuACU---CGUAGACGCG- -5' |
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20775 | 5' | -54.5 | NC_004689.1 | + | 19386 | 0.68 | 0.691483 |
Target: 5'- aUUGCUCAGCgCCAcccUGAGCGaagguggucaucaUCUGuUGCu -3' miRNA: 3'- -AGCGAGUCG-GGUu--ACUCGU-------------AGAC-GCG- -5' |
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20775 | 5' | -54.5 | NC_004689.1 | + | 33878 | 0.68 | 0.670799 |
Target: 5'- aUCGUUCAGCCgCGuuucguucGGCAUUaGCGCa -3' miRNA: 3'- -AGCGAGUCGG-GUuac-----UCGUAGaCGCG- -5' |
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20775 | 5' | -54.5 | NC_004689.1 | + | 25752 | 0.69 | 0.604955 |
Target: 5'- aUCGCgugcCAGCCCcAUGAgaacgucuccgGCGUCUG-GCa -3' miRNA: 3'- -AGCGa---GUCGGGuUACU-----------CGUAGACgCG- -5' |
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20775 | 5' | -54.5 | NC_004689.1 | + | 37146 | 0.69 | 0.604955 |
Target: 5'- -aGCUCAGUCCAGc--GCcgGUCUGCGUc -3' miRNA: 3'- agCGAGUCGGGUUacuCG--UAGACGCG- -5' |
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20775 | 5' | -54.5 | NC_004689.1 | + | 48777 | 0.7 | 0.549548 |
Target: 5'- cUGCUCGGCCUugcGUGccuugcgGGCAUC-GCGCu -3' miRNA: 3'- aGCGAGUCGGGu--UAC-------UCGUAGaCGCG- -5' |
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20775 | 5' | -54.5 | NC_004689.1 | + | 44916 | 0.72 | 0.447508 |
Target: 5'- cCGUUCGGCC--AUGAGCuuGUCUGUGUc -3' miRNA: 3'- aGCGAGUCGGguUACUCG--UAGACGCG- -5' |
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20775 | 5' | -54.5 | NC_004689.1 | + | 25659 | 0.73 | 0.418681 |
Target: 5'- aUCGUauccgUAGCCgGG-GAGCAUCUGCGUu -3' miRNA: 3'- -AGCGa----GUCGGgUUaCUCGUAGACGCG- -5' |
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20775 | 5' | -54.5 | NC_004689.1 | + | 19562 | 1.13 | 0.000758 |
Target: 5'- cUCGCUCAGCCCAAUGAGCAUCUGCGCg -3' miRNA: 3'- -AGCGAGUCGGGUUACUCGUAGACGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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